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Artificial propagation method for xenopus laevis

A technology for artificial reproduction and Xenopus laevis, which is applied in animal husbandry and other directions, can solve problems such as no relevant reports, and achieve the effects of simple and fast operation and reduction of breeding costs.

Active Publication Date: 2022-04-15
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After literature search, there is no relevant report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 1. Build a breeding system

[0059] Establish a breeding pond for frogs and build a breeding system. From November to June of the next year, control the photoperiod at 11 hours, the temperature at 16°C, the flow rate at 60L / min, the water transparency at 55cm, and the dissolved oxygen At 4.6mg / L, the pH was controlled at 7.0.

[0060] 2. Bait preparation

[0061] The raw materials of slurry bait nbf1 are as follows: boiled egg yolk, fish and shrimp, multidimensional element slices, yeast and water, boiled egg yolk: shrimp: multidimensional element slices: yeast: water=250:10:8:2:750 (mass ratio) .

[0062] The raw materials of the mixed bait nbf2 are as follows: boiled egg yolk, fish and shrimp, freshly ground soybean milk, multidimensional element tablets, yeast and water, boiled egg yolk: shrimp: freshly ground soybean milk: multidimensional element tablet: yeast: water = 250:10: 250:8:2:500 (mass ratio).

[0063] The raw materials of the mixed bait nbf3 are as fo...

Embodiment 2

[0084] 1. Build a farming system

[0085] Establish a breeding pond for frogs and build a breeding system. From July to October, the photoperiod is controlled at 13 hours, the temperature is controlled at 120°C, the flow rate is controlled at 110m / min, the water quality transparency is controlled at 85cm, and the dissolved oxygen is controlled at 6.0mg / min. L, the pH value is controlled at 7.2.

[0086] 2. Bait preparation

[0087] The raw materials of slurry bait nbf1 are as follows: boiled egg yolk, fish and shrimp, multidimensional element slices, yeast and water, boiled egg yolk: shrimp: multidimensional element slices: yeast: water=250:10:8:2:750 (mass ratio) .

[0088] The raw materials of the mixed bait nbf2 are as follows: boiled egg yolk, fish and shrimp, freshly ground soybean milk, multidimensional element tablets, yeast and water, boiled egg yolk: shrimp: freshly ground soybean milk: multidimensional element tablet: yeast: water = 250:10: 250:8:2:500 (mass ratio...

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PUM

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Abstract

The invention provides an artificial breeding method for xenopus laevis, and belongs to the technical field of aquatic animal breeding. According to the artificial breeding method for the Xenopus laevis, the Xenopus laevis achieves the purpose of naturally producing sperms and eggs under the conditions of artificial breeding conditions and no use of oxytocin by strictly controlling the environment conditions and the feeding method of parent frog breeding, and the higher fertilization rate and hatching rate can be obtained. The problem that the male and female Xenopus laevis do not develop or do not develop synchronously in a pond culture environment is fundamentally solved; the loss of the breeding frogs of the Xenopus laevis frogs is reduced, particularly the problem that the male frogs are consumed by killing the frogs and taking sperms is solved, and meanwhile, the breeding cost is greatly reduced; the method is more reasonable and efficient, the hatching rate is increased, waste of frog spawn resources is reduced, and the requirements of users for frog spawns in different development periods and tadpoles in different periods are met; through the special hatching and tadpole feeding steps, the hatching rate of fertilized eggs and the survival rate and metamorphosis rate of tadpoles are greatly increased, and meanwhile the metamorphosis rate of tadpoles is also reduced.

Description

technical field [0001] The invention belongs to the technical field of aquatic animal reproduction, and in particular relates to an artificial reproduction method of Xenopus laevis. Background technique [0002] African clawed frog (Xenopus laevis), also known as smooth clawed frog, is an aquatic anuran amphibian native to South Africa, and is an important model organism for studying developmental biology. Xenopus laevis has a maximum body length of up to 120 mm, a flat head and body, and no external ears or tongue. The hind limbs have short 3-toed claws, which are used for digging in mud to avoid predators. Because there is no tongue, it can only use its forelimbs to prey on aquatic animals in the water. Xenopus mainly feeds on small fish, shrimps, crabs and aquatic insects. When looking for food, it often uses the long claws of its forelimbs to continuously push food into its mouth. Lifelong aquatic habitat, widely inhabiting freshwater waters, especially like the envir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCY02A40/81
Inventor 潘晓赋张源伟杨君兴王晓爱吴安丽
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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