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Pleione bulbocodioides tissue rapid propagation method based on somatic embryo approach

A technology of somatic embryos and solitary orchids, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effect of improving the effect

Active Publication Date: 2022-05-06
HANGZHOU ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned problems existing in the prior art, the purpose of the present invention is to design and provide a kind of tissue rapid propagation technical scheme based on the somatic embryo approach, to solve the problem that cannot be obtained from Plant tissues such as leaves, pseudobulbs, and root tips induce the generation of callus, and further tissue culture and propagation of healthy seedlings of Orchid albicans, to achieve rapid and batch expansion of Orchid albicans seedlings

Method used

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  • Pleione bulbocodioides tissue rapid propagation method based on somatic embryo approach
  • Pleione bulbocodioides tissue rapid propagation method based on somatic embryo approach
  • Pleione bulbocodioides tissue rapid propagation method based on somatic embryo approach

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Embodiment 1

[0034] A method for tissue culture and multiplication of Suanlan orchids, comprising the following steps:

[0035] (1) Selection and sterilization of explants.

[0036] The selection of explants: select healthy 3-year-old autumn-flowered orchids, and artificially assist pollination during the flowering period. After the flowers wither and produce young fruit pods, water 0.5% of 20-20-20+Te water-soluble water (Baoruifeng, Haifa, Israel) once, and cover with 70% shade net. Take the green fruit pods of 2-month-old autumn-flowered garlic orchids for tissue culture. At this time, the inner wall tissue of the fruit pods is tender, and the outer skin tissue has not turned completely yellow and fibrous (see figure 1 ).

[0037] Sterilization of explants: Soak the fruit pods in detergent water for 12 minutes, brush the surface of the seed shells with a brush, and rinse them with tap water for 30 minutes; sterilize the surface with 75% alcohol for 40 s on an ultra-clean workbench, 0....

Embodiment 2

[0047] A method for tissue culture and multiplication of Suanlan orchids, comprising the following steps:

[0048] (1) Selection and sterilization of explants.

[0049] Selection of explants: Select healthy 3-4 year old solitary orchid plants, artificially assist pollination of solitary orchids during flowering to increase fruit setting rate, after 1 month of careful cultivation, pick fruit pods, at this time the inner wall of the fruit pods The tissue is tender and the outer skin tissue is not completely fibrotic;

[0050] Sterilization of explants: Soak the fruit pods in detergent water for 10 minutes, brush the surface of the seed shell with a brush, and rinse with tap water for 15 minutes; sterilize the surface with 75% alcohol for 20 s on the ultra-clean workbench, 0.1% Soak and sterilize in mercury chloride for 5 minutes, rinse with sterile water for 5 times, and dry the surface moisture of fruit pods with sterile filter paper;

[0051] (2) Induction culture.

[0052]...

Embodiment 3

[0059] A method for tissue culture and multiplication of Suanlan orchids, comprising the following steps:

[0060] (1) Selection and sterilization of explants

[0061] Selection of explants: Select healthy 3-4 year old Suli orchid plants, carry out artificial assisted pollination to Suli orchid during the flowering period, after 3 months of careful cultivation, pick before the fruit pods mature and turn yellow, the pods are required The outer skin is turquoise, no disease spot or scar, the inner wall tissue of the pod is tender, and the outer skin tissue is not completely fibrous;

[0062] Sterilization of explants: Soak the fruit pods in detergent water for 15 minutes, brush the surface of the seed shells with a brush, and rinse them with tap water for 20 minutes; sterilize the surface with 75% alcohol for 30 s on an ultra-clean workbench, 0.1% Soak and sterilize in mercury chloride for 15 minutes, rinse with sterile water for 6 times, and dry the surface moisture of fruit p...

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Abstract

The invention discloses a pleione bulbocodioides tissue rapid propagation method based on a somatic embryo approach, and belongs to the technical field of tissue culture propagation. The method comprises the following steps: by taking inner wall tissues of pleione bulbocodioides fruit pods 1-3 months after flowering and fruiting as explants and taking a B5 culture medium as a primary culture medium, inducing to form calluses, continuously culturing to form green cluster buds, then carrying out proliferation and propagation by taking MS as a subculture medium, and further differentiating the green cluster buds in form to form protocorm clusters, so as to obtain the pleione bulbocodioides. And when the diameter of the protocorm exceeds 0.5 cm, inducing rooting by adopting a 1 / 2MS rooting culture medium, transplanting well-rooted robust bottle seedlings into a substrate for domestication and cultivation, and finally forming pleione bulbocodioides seedlings. According to the pleione bulbocodioides tissue culture and rapid propagation method, the problems that callus cannot be generated through induction from plant tissues such as leaves, pseudobulb and root tips, and healthy pleione bulbocodioides seedlings cannot be further subjected to tissue culture and propagation can be solved, and rapid and batch propagation of pleione bulbocodioides can be achieved.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and multiplication, and in particular relates to a method for rapid multiplication of garlic orchid tissues based on a somatic embryo approach. Background technique [0002] Rolfe [Pleione bulbocodioides (Franch.) Rolfe] is a perennial herb of the genus Pleione bulbocodioides (Franch.) Rolfe. There are about 20 species in the genus Pleione bulbocodioides, and 16 species are distributed in China. Among them, Pleione bulbocodioides (Franch.) Rolfe are commonly known as ice balls. , together with Rhododendron orchid, is collectively known as Shanzi mushroom, which is a traditional precious Chinese herbal medicine. . Garlic orchid is beautiful in shape, has high ornamental value, and has extremely high medicinal value at the same time, and has a large market demand. With the in-depth research and development of new products, the market demand for Suanlan has been increasing. For a long time, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002Y02A40/22Y02P60/40
Inventor 柴伟国裘劼人童建新周历萍
Owner HANGZHOU ACAD OF AGRI SCI
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