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Cell communication analysis method based on immune cells of patients with primary sicca syndrome

A technology of Sjogren's syndrome and analysis method, which is applied in the field of cell communication analysis based on immune cells of patients with primary Sjögren's syndrome, and can solve problems such as not considering signal cofactors

Pending Publication Date: 2022-05-13
SHENZHEN PEOPLES HOSPITAL
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Problems solved by technology

Based on the above questions, CellPhoneDB v2.0 was developed to predict signaling interactions between two cell populations, however, other important signaling cofactors, including soluble agonists, antagonists, and stimulatory and inhibitory membrane binding co-receptor

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  • Cell communication analysis method based on immune cells of patients with primary sicca syndrome
  • Cell communication analysis method based on immune cells of patients with primary sicca syndrome
  • Cell communication analysis method based on immune cells of patients with primary sicca syndrome

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Embodiment 1

[0053] This example analyzes the cell communication mode of immune cells in the peripheral blood of patients with primary Sjögren's syndrome. The specific process is as follows:

[0054] (1) Collect peripheral blood from patients with primary Sjogren's syndrome: recruit patients with primary Sjögren's syndrome.

[0055] The inclusion criteria are: ① able to actively participate in the trial, with complete basic information; ② symptoms such as pantothenic acid, belching, dysphagia, abdominal distension, arthritis, dry eyes, dry mouth, and swollen lymph nodes.

[0056] Exclusion criteria: ① combined with mental diseases; ② combined with abnormal organ function, liver and kidney diseases, and malignant tumor diseases.

[0057] According to the "2016 American College of Rheumatology / European League Against Rheumatism Classification Criteria for Primary Sjogren's Syndrome" and "Primary Sjögren's Syndrome Diagnosis and Treatment Standards" diagnosis.

[0058] All patients signed in...

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Abstract

The invention belongs to the technical field of biological detection, and discloses a cell communication analysis method based on immune cells of a patient with primary sicca syndrome. The cell communication analysis method comprises the following steps: separating immune cells in peripheral blood of the patient, preparing a single-cell suspension, carrying out single-cell transcriptome sequencing, and finally analyzing an intercellular communication mode by using CellChat software. According to the analysis method provided by the invention, the known structural composition of ligand-receptor interaction is fully considered, cell communication is combined with a cell function pathway, and then single cell transcriptome sequencing is combined for comprehensive analysis; a brand new thought is provided for searching treatment targets of diseases related to the primary sicca syndrome and exploring a regulation and control mechanism between cell communication and cell biological functions.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a cell communication analysis method based on immune cells of patients with primary Sjögren's syndrome. Background technique [0002] Primary Sjogren's syndrome (pSS) is a chronic inflammatory autoimmune disease characterized by lymphocyte proliferation and progressive exocrine gland damage. There are a variety of autoantibodies in the serum of patients, and the incidence rate is 0.5 %~1.0%, of which more than 90% are women. The clinical manifestations are dry mouth, dry eyes, joint pain, fatigue and other discomfort symptoms. About 1 / 3 of the patients have multi-system damage such as lung, kidney, nervous system, blood vessel or lymphatic system. The exact etiology and pathogenesis are still unknown. Not very clear, is a refractory disease. [0003] Cell communication refers to the process in which a signal sent by one cell is transmitted to another ce...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/6888G16B25/00
CPCC12Q1/6869C12Q1/6888G16B25/00C12Q2535/122
Inventor 戴勇侯显良汤冬娥洪小平刘冬舟蔡晚霞
Owner SHENZHEN PEOPLES HOSPITAL
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