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Walnut polypeptide with good emulsifying property and preparation method thereof

A technology of walnut peptides and emulsifying properties, which is applied in the fields of peptide preparation methods, chemical instruments and methods, organic chemistry, etc., to achieve the effects of increasing economic benefits, improving utilization rates, and broadening application fields

Active Publication Date: 2022-06-10
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a walnut polypeptide with good emulsifying properties and stability through restricted enzymolysis of walnut protein, and to provide a preparation method to solve the problems of complex preparation process and low emulsifiability of existing walnut polypeptides

Method used

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  • Walnut polypeptide with good emulsifying property and preparation method thereof
  • Walnut polypeptide with good emulsifying property and preparation method thereof
  • Walnut polypeptide with good emulsifying property and preparation method thereof

Examples

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Embodiment 1

[0024] 1. Extraction of walnut protein

[0025] (1) Degreasing: Pass the walnut meal through a 100-mesh sieve, add it to petroleum ether at a material-to-liquid ratio of 1 g: 5 mL, degrease at room temperature for 2 hours, repeat the degreasing 3 times, and dry naturally overnight to obtain the defatted walnut meal.

[0026] (2) Alkaline extraction and acid precipitation: According to the ratio of material to liquid: 1g: 15mL, add defatted walnut meal into deionized water, adjust the pH value of the solution to 11 with 1mol / L NaOH aqueous solution, and stir magnetically at 53°C for 1.5h to make the protein fully Dissolve, cool to room temperature and centrifuge at 5000r / min for 5min, adjust the pH value of the supernatant to 4.5 with 1mol / L HCl aqueous solution, let it settle for 1h, then centrifuge, wash the precipitate with deionized water until neutral, and freeze-dry in vacuum After that, walnut protein is obtained.

[0027] 2. Enzymatic hydrolysis of walnut protein

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Abstract

The invention discloses a walnut polypeptide with a good emulsifying property and a preparation method of the walnut polypeptide. The walnut polypeptide is a polypeptide mixture of which the amino acid sequences are Glu-Lys-Val-Ser-Tyr-Asp-His-Cys-Ile-Met-Ser-Leu-Asp, Gly-Gln-Phe-His-Ala-Trp-Lys-Asn-Pro-Asn-Thr-Met-Ser and Asp-Glu-His-Val-Leu-Lys-Phe-Gly-Met-Thr-Cys. The invention further discloses a preparation method of the walnut polypeptide with the good emulsifying property. The method comprises the following steps: by taking walnut meal as a raw material, degreasing, performing alkali extraction and acid precipitation, performing restriction enzymolysis by using trypsin, and finally performing ultrafiltration separation and sephadex G-25 gel chromatography separation and purification to obtain the walnut polypeptide. The molecular weight of the walnut polypeptide ranges from 3 kDa to 5 kDa, the emulsifying property and the emulsifying stability of the walnut polypeptide are good, a theoretical basis is provided for development of polypeptide emulsifiers, the application field of walnut protein is widened, meanwhile, waste can be turned into wealth, the utilization rate of the walnut protein is increased, the industrial application of the walnut protein is enriched, and the economic benefits of the walnut industry are increased.

Description

technical field [0001] The invention belongs to the technical field of polypeptide products, and in particular relates to a walnut polypeptide with good emulsifying performance and a preparation method thereof. Background technique [0002] my country is the country with the largest walnut cultivation area, the most planted trees and the highest yield in the world. In 2018, my country's walnut production reached 1.5863 million tons, accounting for 45% of the world's total walnut production. Walnut meal is a by-product of walnut oil extraction, and the defatted walnut meal contains 50% to 60% protein. Walnut protein not only contains 8 kinds of essential amino acids for human body, but also is rich in semi-essential amino acids. At present, walnut meal is mainly used as feed and fertilizer, or is discarded directly, resulting in the waste of a large amount of high-quality walnut protein resources, and the utilization rate and economic benefits are relatively low. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K7/06C07K7/08C07K1/30C07K1/34C07K1/22C07K1/36C07K1/14
CPCC12P21/06C07K7/06C07K7/08Y02P60/87
Inventor 孟永宏师琳珊李封辰杨雪言张帅路亚龙
Owner SHAANXI NORMAL UNIV
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