Microbial fertilizer with function of promoting growth of tobacco plants as well as preparation method and application of microbial fertilizer

A technology of microbial fertilizer and compound microbial bacteria, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as differences in resistance to diseases and insect pests, uneven growth and development of tobacco, and improve nutritional resistance. , Improve the absorption area and specific surface area, and promote the effect of growth and development

Pending Publication Date: 2022-06-24
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to propose a microbial fertilizer with the function of promoting the growth of tobacco plants, a preparation method and its application, which solves the technology of uneven growth and development of tobacco and differences in the ability to resist diseases and insect pests in the production process of tobacco leaves in the prior art question

Method used

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  • Microbial fertilizer with function of promoting growth of tobacco plants as well as preparation method and application of microbial fertilizer
  • Microbial fertilizer with function of promoting growth of tobacco plants as well as preparation method and application of microbial fertilizer
  • Microbial fertilizer with function of promoting growth of tobacco plants as well as preparation method and application of microbial fertilizer

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Experimental program
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Effect test

Embodiment 1

[0031] The microbial fertilizer with the function of promoting the growth of tobacco plants in the present embodiment is prepared in the following manner:

[0032] S1: Strain activation. Inoculate 0.1 ml of the Bacillus amyloliquefaciens YWF-17-0001 and Bacillus subtilis D23 preservation solutions in glycerol at -80 °C into 10 ml of beef extract peptone liquid medium at a temperature of 30 °C and a rotation speed of 150 rpm Activated and cultured for 48 hours under the conditions to obtain the activated culture solution of Bacillus amyloliquefaciens YWF-17-0001 strain and Bacillus subtilis strain D23.

[0033] S2: strain purification. Use a sterilized inoculation loop to inoculate the bacteria in the activated culture solution on the beef extract peptone solid medium plate, carry out purification and streak culture, and cultivate in an incubator with a temperature of 33 ° C for 3 days. Select the colony characteristics that meet the functional bacteria. A single larger colon...

Embodiment 2

[0044] The microbial fertilizer with the function of promoting the growth of tobacco plants in the present embodiment is prepared in the following manner:

[0045] S1: Strain activation. Inoculate 0.1 ml of the Bacillus amyloliquefaciens YWF-17-0001 and Bacillus subtilis D23 preservation solutions in glycerol at -80 °C into 10 ml of beef extract peptone liquid medium at a temperature of 35 °C and a rotation speed of 150 rpm. The activated culture solution of Bacillus amyloliquefaciens YWF-17-0001 strain and Bacillus subtilis strain D23 was obtained by activating culture under conditions for 42 hours.

[0046] S2: strain purification. Use a sterilized inoculation loop to inoculate the bacteria in the activated culture solution on the beef extract peptone solid medium plate, carry out purification and streak culture, and cultivate in an incubator with a temperature of 35 ° C for 2 days, and select the colony characteristics that meet the functional bacteria. A single larger co...

Embodiment 3

[0057] The microbial fertilizer with the function of promoting the growth of tobacco plants in the present embodiment is prepared in the following manner:

[0058] S1: Strain activation. Inoculate 0.1 ml of the Bacillus amyloliquefaciens YWF-17-0001 and Bacillus subtilis D23 preservation solutions in glycerol at -80 °C into 10 ml of beef extract peptone liquid medium at a temperature of 37 °C and a rotation speed of 150 rpm. Activated and cultured for 36 hours under the conditions to obtain the activated culture solution of Bacillus amyloliquefaciens YWF-17-0001 strain and Bacillus subtilis strain D23.

[0059] S2: strain purification. Use a sterilized inoculation loop to inoculate the bacteria in the activated culture solution on the beef extract peptone solid medium plate, carry out purification and streak culture, and culture in an incubator with a temperature of 37 ° C for 1 day, and select the colony characteristics that meet the functional bacteria A single larger colo...

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Abstract

The invention discloses a microbial fertilizer with a function of promoting growth of tobacco plants as well as a preparation method and application of the microbial fertilizer. The preparation method of the microbial fertilizer with the function of promoting the growth of the tobacco plants comprises the following steps: respectively preparing fermentation broth of bacillus amyloliquefaciens YWF-17-0001 and fermentation broth of bacillus subtilis D23; mixing the fermentation liquor of the bacillus amyloliquefaciens YWF-17-0001 and the fermentation liquor of the bacillus subtilis D23 to prepare a compound microorganism bacterium solution with the function of promoting the growth of tobacco plants; and mixing the compound microbial bacterial liquid with an auxiliary agent to prepare the microbial bacterial fertilizer with the function of promoting the growth of tobacco plants, wherein the effective viable count in each gram of the microbial bacterial fertilizer is 2.0 * 10 < 8 > cfu/g-4. 0 * 10 < 8 > cfu/g. The microbial fertilizer disclosed by the invention is applied as a base fertilizer, has rooting and root strengthening effects on tobacco plants, and can improve the rooting quantity of the tobacco plants and promote the root system to develop robustly, so that the nutrient absorption of the tobacco plants is improved, the risk of root diseases caused by weak root system development of the tobacco plants is reduced, the nutrition resistance of the tobacco plants is improved, and the production quality and yield of tobacco leaves are ensured.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizers, in particular to a microbial fertilizer with the function of promoting the growth of tobacco plants, a preparation method and applications thereof. Background technique [0002] Tobacco is one of the important foliar economic crops in China, and its growth and development, nutrient acquisition and quality formation are closely related to its microbiological characteristics and soil environment. The main functions of microbial fertilizers in tobacco planting are as follows: (1) assisting the absorption of nutrients by tobacco; (2) improving tobacco planting soil and improving the agricultural ecological environment; (3) improving the yield and quality of tobacco leaves; ( 4) Improve the ability of tobacco to resist pests and diseases. [0003] In recent years, Yunnan tobacco has been affected by drought from time to time. In addition, there are differences in tobacco varieties, seed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/07C12R1/125
CPCC12N1/20C05G3/00C05C11/00C05D5/00C05D9/00C05F5/002C05F11/08
Inventor 孙军伟尚海丽范志勇户艳霞王德勋李娟余源何海萍苏家恩蒯雁盘文政王斌张琪
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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