Long non-coding RNALINC01963 as lung cancer tumor marker and therapeutic target

A LINC01963, non-coding technology, applied in the fields of biomedicine and molecular biology, can solve problems such as unclear effect, achieve high accuracy and promote apoptosis

Pending Publication Date: 2022-06-24
浙江百越生物技术有限公司
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Problems solved by technology

However, the role of LINC01963

Method used

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  • Long non-coding RNALINC01963 as lung cancer tumor marker and therapeutic target
  • Long non-coding RNALINC01963 as lung cancer tumor marker and therapeutic target
  • Long non-coding RNALINC01963 as lung cancer tumor marker and therapeutic target

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 2: The effect of LINC01963 expression on the biological behavior of lung cancer cells

[0034] (1) Determination of cell transfection efficiency: In order to regulate the level of LINC01963 in lung cancer cells, short hairpin RNA (shLINC01963; 5'-CTCTCCCTACACCAGAATTTA-3') and LINC01963 overexpression plasmid were transfected into H1299 and A549 cells through Lipo 3000 middle. Empty vector was used as a negative control. After trypsinization of cells, cells were seeded at 2 x 105 cells / well in 24-well plates and grown to 70-90% confluency. Dilute Lipo 3000 (0.75 µL) and sample (1 µg) in Opti-MEM medium (25 µL x 2), then add 2 µL of P3000 reagent to the diluted sample. The sample-reagent mixture was then mixed with diluted Lipo 3000 (1:1) and incubated at room temperature for 10 minutes. Finally, complexes (50 µL / well) were added to cells and incubated at 37°C for 24 hours. Then qRT-PCR was performed as in Example 1(1). The result is as figure 2 As shown in...

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Abstract

The invention belongs to the field of biological medicine, particularly belongs to the technical field of molecular biology, and particularly relates to application of long non-coding RNA LINC01963 as a lung cancer tumor marker and a treatment target. The invention provides a long non-coding RNA LINC01963 as a lung cancer tumor marker and a treatment target, the long non-coding RNA LINC01963 is low in expression in a lung cancer patient, the expression of the long non-coding RNA LINC01963 is remarkably related to lymph node metastasis and clinical staging of the patient, the long non-coding RNA LINC01963 as a lung cancer diagnosis marker has high accuracy, overexpression of the LINC01963 inhibits lung cancer cell proliferation, migration, invasion and tube formation, and the long non-coding RNA LINC01963 has a good application prospect. And the apoptosis of lung cancer cells is promoted, so that the gene can become an important target for lung cancer gene therapy.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to the technical field of molecular biology, and particularly relates to the application of long non-coding RNALINC01963 as a lung cancer tumor marker and a therapeutic target. Background technique [0002] Lung cancer is considered the number one cause of cancer-related mortality in men worldwide and the fourth leading cause of mortality in women. Global cancer statistics in 2018 showed that there were 2,093,876 new lung cancer cases and 1,761,007 deaths, accounting for 11.6% of new cancer cases and 18.4% of cancer-related deaths. In recent years, despite great efforts in chemotherapy, radiotherapy, surgery, immunotherapy and targeted therapy, poor prognosis accompanied by drug resistance and recurrence are still common. Since 65% of lung cancer cases are classified as intermediate and advanced at the time of initial diagnosis, the 5-year survival rate of patients is only 4-17%. Theref...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11A61K31/7105A61P35/00A61P11/00
CPCC12Q1/6886A61K31/7105A61P35/00A61P11/00C12Q2600/158C12Q2600/178C12Q2600/112C12Q2600/118
Inventor 曹卓潘炯伟叶再挺蔡晓平尹章勇徐存来
Owner 浙江百越生物技术有限公司
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