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Method for determining vigor detection indexes of purple perilla seeds

A technology for vigor detection and perilla seeds, which is applied in the field of determination of perilla seed vigor detection indicators, and can solve the problem of high cost

Active Publication Date: 2022-07-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this meter is very portable, it has the problem of high cost

Method used

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  • Method for determining vigor detection indexes of purple perilla seeds
  • Method for determining vigor detection indexes of purple perilla seeds
  • Method for determining vigor detection indexes of purple perilla seeds

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Experimental program
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Embodiment 1

[0050] The present embodiment provides a method for determining a perilla seed vigor detection index, comprising the following steps:

[0051] (1) Scan 600 perilla seeds on a Microtek MiCardWizard scanner to obtain an image of the seed epidermis, and extract the visual phenotype index data of the seed coat through the seed phenotype automatic extraction system (PhenoSeed);

[0052] (2) The perilla seeds are germinated, and the method for germination is specifically: first, soak each seed in clear water for 24h in order, change the water every 2h, and then carry out a germination test on paper in the germination box: Germination A 1cm thick sponge is placed at the bottom of the box, a layer of germination paper is placed on the top as a germination bed, and then the seeds are placed in the germination box in order, 600 seeds are placed in 6 boxes, 100 seeds in each box, and placed in a 25°C light incubator Germination was carried out within the 8th day, and the germination stat...

Embodiment 2

[0058] The present embodiment provides a method for determining a perilla seed vigor detection index, comprising the following steps:

[0059] (1) Scan 500 perilla seeds on a Microtek MiCardWizard scanner to obtain an image of the seed epidermis, and extract the visual phenotype index data of the seed coat through the seed phenotype automatic extraction system (PhenoSeed);

[0060] (2) The perilla seeds are germinated, and the method of germination is specifically: first, soak each seed in clear water for 20h in sequence, change the water every 2h, and then carry out a germination test on paper in the germination box: Germination A 1cm thick sponge is placed at the bottom of the box, and a layer of germination paper is placed on top as a germination bed, and then the seeds are placed in the germination box in order, 500 seeds are placed in 5 boxes, 100 seeds in each box, and placed in a light incubator at 28°C Germination was carried out within the 6th day, and the viability o...

Embodiment 3

[0066] The present embodiment provides a method for determining a perilla seed vigor detection index, comprising the following steps:

[0067] (1) Scan 700 perilla seeds on a Microtek MiCardWizard scanner to obtain an image of the seed epidermis, and extract the visual phenotype index data of the seed coat through the seed phenotype automatic extraction system (PhenoSeed);

[0068] (2) Germination of perilla seeds is carried out, and the method of germination treatment is specifically: first, soak each seed in clear water for 30h in order, change the water every 3h, and then carry out germination test on paper in the germination box: Germination A 1cm thick sponge is placed at the bottom of the box, and a layer of germination paper is placed on top as a germination bed, and then the seeds are placed in the germination box in order, 700 seeds are placed in 7 boxes, 100 seeds in each box, and placed in a 22°C light incubator Germination is carried out within the 10th day, and th...

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Abstract

The invention relates to the technical field of traditional Chinese medicine identification, and provides a method for determining vigor detection indexes of purple perilla seeds, which comprises the following steps: scanning the purple perilla seeds on a scanner to obtain phenotypic indexes of seed coats; germinating the purple perilla seeds and measuring the seed viability; performing correlation analysis on the phenotypic indexes and the viability to obtain correlation coefficients of the phenotypic indexes and the viability of the seeds; screening out the indexes of which the absolute values of the correlation coefficients are greater than or equal to 0.4 to obtain initial seed vigor detection indexes; selecting seeds from different sources and in different processing links to screen the preliminary seed vigor detection indexes, and determining the final seed vigor rapid detection index. According to the method, the vigor of the purple perilla seeds can be quickly judged by detecting the a value of the seed epidermis, so that the method is used for quickly comparing the quality of the seeds and comparing whether the processing procedure is effective or not and whether the seed treatment is effective or not. Compared with chlorophyll determination, the method has the advantages of lower economic cost, simplicity, convenience, rapidness, high stability and wide application value.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine identification, in particular to a method for determining a perilla seed vigor detection index. Background technique [0002] Perilla frutescens (L.) Britt.) is an annual herb of the Lamiaceae family and is one of the traditional Chinese medicinal materials in my country. . In order to meet market demand, my country has begun large-scale standardized artificial cultivation of perilla. However, most of the perilla seeds currently on the market are directly harvested from the field or wild, and the seed clarity, seed emergence rate and seedling uniformity are low. Two standards related to the quality of perilla seeds, namely the agricultural industry standard of the People's Republic of China NY / T 2494-2013 and the local standard of Hebei Province DB13 / T 1320.1-2010, the specified clarity standard (≥95%) and germination rate standard ( ≥75%) are far below the quality standard ...

Claims

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Application Information

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IPC IPC(8): A01C1/02
CPCA01C1/025
Inventor 孙群侯浩楠吴伟锋许亚男曹海禄董学会
Owner CHINA AGRI UNIV