Cooked millet prolamin peptides inhibiting alpha-amylase and alpha-glucosidase

A technology of glucosidase and gliadin, applied in the direction of peptide/protein component, peptide, peptide source, etc., can solve the problem that there is no research on millet protein hypoglycemic peptide, and achieve the effect of good potential and application prospect.

Pending Publication Date: 2022-07-08
CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, antioxidant, anti-inflammatory and lipase inhibitory peptides have been isolated fr

Method used

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  • Cooked millet prolamin peptides inhibiting alpha-amylase and alpha-glucosidase
  • Cooked millet prolamin peptides inhibiting alpha-amylase and alpha-glucosidase
  • Cooked millet prolamin peptides inhibiting alpha-amylase and alpha-glucosidase

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] The extraction of embodiment 1 cooked millet prolamin

[0028] First grind the millet into powder and pass it through a 60 mesh sieve. Then, the millet powder was dispersed in n-hexane at a ratio of 1:5 (w / v), shaken in a water bath at 37°C for 4 hours, and then left to stand. After the millet powder and n-hexane were clearly separated, the upper n-hexane was poured out and the lower layer was collected The product was dried in a fume hood for 24 hours to completely remove n-hexane, and the obtained millet flour was raw millet flour. Mix the defatted raw millet flour with distilled water in a ratio of 1:5. After gelatinization in a boiling water bath for 10min, the precipitate is dried in an oven at 43°C for 24h, then powdered and sieved through a 60-mesh sieve. millet powder. Disperse the cooked millet flour in a 70% ethanol solution at a ratio of 1:7 (m / v), then set the temperature to 40° C. and shake in a water bath shaker for 2 hours. Then, the above reaction sol...

Example Embodiment

[0029] Example 2 In vitro simulated digestion of cooked millet prolamin

[0030] Mix the cooked millet prolamin sample with distilled water at a ratio of 1:5, adjust the pH to 3, add 2000U / mL pepsin, adjust the pH to 7 after the reaction for 2 hours, and then add trypsin to make the pancreatin activity in the digestive juice as 100U trypsin / mL digestion solution, continue the reaction for 2h, after the reaction is over, the enzyme is inactivated in a boiling water bath for 10min to terminate the reaction. Finally, the precipitate was collected by centrifugation at 7000rpm for 10min, and the hydrolyzate after protein digestion was obtained.

Example Embodiment

[0031] Embodiment 3 The ultrafiltration of cooked millet prolamin in vitro simulated digestion product

[0032] First pre-clean with ultrapure water Ultra-15 centrifugal filter, washed and dried. Then select a centrifugal filter with a separation molecular weight of 3kDa, add no more than 15mL of sample to the filter, put the filter device with the lid into the centrifugal rotor, rotate at a speed of 5000×g, and rotate for about 30min. After centrifugation, remove the cap and filter, collect the liquid in the centrifuge tube and freeze-dried, which is the ultrafiltration sample of protein hydrolysate less than 3kDa.

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Abstract

The invention provides cooked millet prolamine peptide for inhibiting alpha-amylase and alpha-glucosidase as well as an application and a screening method of the cooked millet prolamine peptide. The sequence of the cooked millet prolamine peptide is selected from SEQ ID NO.1-6. The peptide can effectively inhibit the activity of alpha-glucosidase and alpha-amylase at the same time, is safe and free of toxic and side effects, and therefore has good potential and application prospects when being used as a functional component in food, health care products and hypoglycemic drugs.

Description

technical field [0001] The present application belongs to the field of proteins, and specifically, the present application provides a plurality of cooked millet prolamin peptides that inhibit α-amylase and α-glucosidase, and methods for their application and screening. Background technique [0002] Diabetes mellitus has become a global prevalent metabolic disease, mainly manifested as the disorder of body fat, carbohydrate and protein metabolism caused by elevated blood sugar levels. The prevalence of diabetes has been steadily increasing in recent years. Non-insulin-dependent diabetes mellitus, or type 2 diabetes, commonly occurs in people of all ages, and 90% of diabetic patients have been diagnosed with type 2 diabetes. Dietary starch can be digested by alpha-amylase into large amounts of maltose and then by alpha-glucosidase into glucose. Elevated blood sugar levels caused by the rapid breakdown of starch into glucose is known as postprandial hyperglycemia, an importan...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12P21/06C07K1/34A61K38/16A61P3/10A23L33/18G01N27/626
CPCC07K14/415C12P21/06A61P3/10A23L33/18G01N27/626A61K38/00A23V2002/00A23V2200/328A23V2250/55
Inventor 沈群付永霞赵卿宇王超薛勇赵亮星牛雁
Owner CHINA AGRI UNIV
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