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Plant growth promoter for water purification, preparation method and application

A technology of plant growth promotion and growth promoter, which is applied in the field of environmental restoration, can solve the problems of elongated purification process, slow plant growth, and reduced efficiency, and achieve the effect of promoting water body restoration, promoting growth and development, and high-efficiency purification

Inactive Publication Date: 2022-07-12
XUZHOU COLLEGE OF INDAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological method can generally purify the water body through microorganisms and water purification plants, and can form a small ecosystem. It is not only efficient, but also does not cause secondary pollution to the environment. However, due to the slow growth of plants, the purification process is elongated and the efficiency is low. reduce

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present embodiment provides a water purification plant growth promoter and a preparation method thereof

[0031] The preparation steps of the growth promoter are as follows:

[0032] S1, preparation of algae extract: Weigh chlorella, kelp and seaweed respectively according to the mass ratio of 5:3:3, mix and soak in water at 50°C for 2 hours, place at 0°C for 10 minutes, and then transfer to 100°C Hold for 10min, repeat the above steps 3 times, then ultrasonicate for 1.5min at 400W, centrifuge at 4°C and 4000rpm for 15min, collect the supernatant, and obtain the algae extract;

[0033] Preparation of microbial additives: activated Bacillus cereus by plate streaking, inoculated into liquid broth medium, and cultivated to OD at 37°C 600 When it is 0.7, inoculate it into fresh broth medium according to the inoculum volume fraction of 2%, and cultivate it at 37°C until the number of viable bacteria of Bacillus cereus is 5ⅹ10 8 CFU / mL, to obtain Bacillus cereus inoculu...

Embodiment 2

[0039] The present embodiment provides a water purification plant growth promoter and a preparation method thereof

[0040] The preparation steps of the growth promoter are as follows:

[0041] S1, preparation of algae extract: Weigh chlorella, kelp and seaweed respectively according to the mass ratio of 4:3:3, mix and soak in water at 55°C for 2 hours, place at 0°C for 4 minutes, and then transfer to 100°C Keep for 15min, repeat the above steps twice, then ultrasonicate for 1min at 410W, centrifuge at 4°C and 4000rpm for 20min, collect the supernatant to obtain the algae extract;

[0042] Preparation of microbial additives: activated Bacillus cereus by plate streaking, inoculated into liquid broth medium, and cultivated to OD at 37°C 600 When it is about 0.7, inoculate it into fresh broth medium according to the inoculum volume fraction of 2.5%, and cultivate it at 37 °C until the number of viable bacteria of Bacillus cereus is 4ⅹ10 8 CFU / mL, to obtain Bacillus cereus inocu...

Embodiment 3

[0048] The present embodiment provides a water purification plant growth promoter and a preparation method thereof

[0049] The preparation steps of the growth promoter are as follows:

[0050] S1, preparation of algae extract: Weigh chlorella, kelp and seaweed respectively according to the mass ratio of 6:3:3, mix and soak in water at 60°C for 2 hours, place at 0°C for 7 minutes, and then transfer to 100°C Keep for 20min, repeat the above steps 3 times, then ultrasonicate at 420W for 2min, centrifuge at 4°C and 4600rpm for 10min, collect the supernatant to obtain the algae extract;

[0051] Preparation of microbial additives: activated Bacillus cereus by plate streaking, inoculated into liquid broth medium, and cultivated to OD at 37°C 600 When it is about 0.7, inoculate it into fresh broth medium according to the inoculum volume fraction of 3%, and cultivate at 37 °C until the number of viable bacteria of Bacillus cereus is 6ⅹ10 8 CFU / mL, to obtain Bacillus cereus inoculum...

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Abstract

The invention relates to the technical field of environmental remediation, and particularly discloses a water body purification plant growth promoter, a preparation method and application, the growth promoter is prepared from the following components in parts by weight: compound sodium nitrophenolate, an algae extracting solution and a microbial additive, the algae extracting solution is prepared by mixing and extracting chlorella, kelp and laver, and the microbial additive is prepared by mixing and extracting chlorella, kelp and laver. The microbial additive is formed by mixing a bacillus cereus microbial agent and a bacillus licheniformis microbial agent according to the volume ratio of 5: (2-3), and the viable count of the bacillus cereus microbial agent and the viable count of the bacillus licheniformis microbial agent are both 4-6 * 10 < 8 > / mL. The growth promoter prepared by the invention can promote the growth of water body purification plants, and can cooperate with the water body purification plants to purify the water body and reduce the pollutants in the water body.

Description

technical field [0001] The invention relates to the technical field of environmental restoration, in particular to a water purification plant growth promoter, a preparation method and an application. Background technique [0002] When the pollutants entering the water body exceed the environmental capacity of the water body or the self-purification capacity of the water body, the water quality deteriorates, thereby destroying the original value and function of the water body, which is called water pollution. Special geological conditions make a large amount of chemical elements enriched, natural plants produce some harmful substances when they rot, and water pollution caused by rainwater falling to the ground and carrying various substances into the water body are all natural pollution. Of course, a large part of water pollution is also caused by humans. Improper treatment of industrial wastewater and domestic sewage, or direct discharge without treatment, will cause water ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N33/22A01N65/03A01N63/22C02F3/32C12N1/20C12R1/085C12R1/10
CPCA01N33/22A01N65/00A01N65/03A01N63/22C02F3/32C12N1/20Y02W10/10
Inventor 李秀芳吴建堃曹倩
Owner XUZHOU COLLEGE OF INDAL TECH
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