Cultivation method of dual-purpose genic male sterile line with low sterility starting temperature of rice

Abstract

The invention discloses a cultivation method of a dual-purpose genic male sterile line with low sterility starting temperature of rice, and belongs to the field of crop breeding. According to the method, two generations of sterile single plants F3 and F5 are screened under the condition that the sunshine length of Hainan Island in autumn and winter is shortened, and the dual-purpose genic male sterile line with the low sterile starting point temperature of rice with the sterile starting point temperature not higher than 23 DEG C is screened in combination with F4 generation in-season fertility evaluation and a fertility identification result of a third-party detection mechanism. According to the method, the defect that the cultivation of the two-line sterile line depends on low-temperature pool screening is overcome, the dual-purpose genic male sterile line with the low sterile starting point temperature is successfully cultivated, and the safe application of the dual-purpose genic male sterile line and the healthy development of two-line hybrid rice are promoted.

Description

technical field [0001] The invention relates to the field of crop breeding, in particular to a method for cultivating a dual-purpose nuclear sterile line of rice with low sterile starting point and temperature. Background technique [0002] Two-line hybrid rice is an original high-tech in China, which mainly focuses on the utilization of photothermal-sensitive sterile lines. It is another major technological innovation in rice genetics and breeding after three-line hybrid rice. Low cost, high yield advantage, relatively superior quality and other technical advantages. From 1994 to 2019, 882 two-line rice varieties were approved by the state, and 1,604 were approved at the provincial level. The two-line rice varieties approved from 2016 to 2019 accounted for 58.9% of the approved hybrid rice varieties, of which the two-line medium indica varieties accounted for 64.7% of the medium indica hybrid rice varieties. However, the two-line sterile line is affected by light and temp...

AI Technical Summary

Problems solved by technology

However, the two-line CMS is affected by light and temperature factors, and the CMS with a higher starting point of sterility is affected by the inter-annual climate, so the risk of seed production is high, which restricts the application of two-line hybrid rice to a certain extent.

In order to ensure the safety of seed production, the key is to cultivate two-line CMS lines with low sterility threshold temperature, but the cultivation of dual-purpose GMS lines with low sterility threshold temperature generally depends on the selection of low-temperature pools, which requires large investment, high operating costs, and Technically difficult and highly dependent on equipment

Often, many two-line male sterile lines are not able to be screened in low-temperature pools, resulting in a higher starting temperature for sterile lines, which restricts the safe application of dual-purpose nuclear male sterile lines and the healthy development of two-line hybrid rice.