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Microwell plate for containing plurality of samples

A microplate and sample technology, applied in the field of microplate, can solve the problems of reducing experimental output and so on

Pending Publication Date: 2022-07-29
LEICA MICROSYSTEMS CMS GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Additionally, moving samples from one microplate to another by pipetting reduces assay throughput

Method used

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  • Microwell plate for containing plurality of samples
  • Microwell plate for containing plurality of samples
  • Microwell plate for containing plurality of samples

Examples

Experimental program
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Embodiment Construction

[0031] figure 1 A schematic top view and corresponding cross-sectional view of the microplate 100 are shown. Microplate 100 includes 192 sample chambers 102 arranged in an 8 by 24 grid. Each cavity 102 includes a donor compartment 104 and an acceptor compartment 106 . Thus, the microplate 100 includes 384 compartments 104 , 106 . Alternatively, the microplate 100 may have additional or fewer sample cavities 102 .

[0032] Each of the compartments 104 , 106 is open towards the top side 108 of the microplate 100 and includes openings, in particular the donor compartment 104 includes the donor opening 110 and the acceptor compartment 106 includes the acceptor opening 112 . The openings 110, 112 allow individual access to the interior of the respective compartments 104, 106, eg, for individually adding or removing samples using a manual pipette or using a liquid handling robot. figure 1 The openings 110, 112 of the microplate 100 are rectangular in shape. Similarly, the side ...

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Abstract

A microwell plate (100, 400) for containing a plurality of samples (612), comprising a plurality of sample cavities (102), each sample cavity (102) comprising at least one separating element (120, 402) separating the sample cavity (102) into at least one donor compartment (104) and an acceptor compartment (106). The partition element (120, 402) is configured to receive one of the samples within one of the donor compartment (104) or the acceptor compartment (106) when the microwell plate (100, 400) is in the first orientation. The separating element (120, 402) is further configured to allow the sample (612) to be transferred at least from the donor compartment (104) to the acceptor compartment (106) of one of the sample cavities (102) when the microplate (100, 400) is in the second orientation. Another aspect is a method for transferring a sample between compartments (104, 106) of a microwell plate (100, 400).

Description

technical field [0001] The present invention relates to a microplate for containing a plurality of samples. Furthermore, the present invention relates to a method for transferring a sample. Background technique [0002] Microplates are frequently used in research and development, especially in the life sciences and pharmaceutical industries. Microplates have specific applications in the fields of high-throughput in vitro cell culture, cell assays, and cell analysis. More recently, suspension 3D cell culture methods have been developed that allow the cultivation of cells and cell clusters, such as spheroids or organoids, in microplates. Microplates typically include multiple chambers for culturing spheroids individually under different experimental conditions. Similar to culture, cell assays and cell analysis benefit from the use of microplates: increased experimental throughput through parallel high-throughput workflows. Typically, these cellular assays and cellular anal...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N35/00
CPCB01L3/5027B01L3/502715G01N35/00029B01L2200/0636B01L2300/0829B01L2300/0848B01L2300/0893C12M23/12C12M21/08C12M23/34C12M23/02C12M29/00B01L3/50853B01L2200/0689B01L2200/16B01L2200/0652B01L3/5085B01L2400/086B01L9/523G01N35/028G01N2035/00287G01N2035/0403
Inventor 索伦·阿尔斯海默
Owner LEICA MICROSYSTEMS CMS GMBH
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