Moringa seed extract with anti-influenza virus activity

An anti-influenza virus, anti-viral activity technology, applied in antiviral agents, sugar derivatives, organic chemistry, etc., can solve the problem of influenza virus gene reassortment efficiency vaccine escape, vaccine cannot be eradicated, difficult to deal with changeable influenza viruses, etc. question

Pending Publication Date: 2022-07-29
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing anti-flu drugs struggle to cope with changing flu viruses
Although vaccines are the core drugs for the prevention and treatment of viral infections in humans, certain zoonotic coronaviruses cannot be eradicated by vaccines for humans. At the same time, the high gene reassortment efficiency of influenza viruses makes them escape from vaccines. Phenomenon

Method used

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  • Moringa seed extract with anti-influenza virus activity
  • Moringa seed extract with anti-influenza virus activity
  • Moringa seed extract with anti-influenza virus activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] like Figures 1 to 6 Shown, a kind of Moringa seed extract with anti-influenza virus activity, described Moringa seed extract is named Moringa A, and its chemical structure is:

[0022]

[0023] Preferably, the Moringa seed extract Moringa A is obtained from the Moringa seed methanol extract-acetone extract phase.

[0024]Preferably, the preparation method of the Moringa seed extract Moringa A: 30 kg of dried Moringa seeds are crushed to fine powder, and extracted twice with methanol for 2 hours each time; Methanol was recovered, and the crude extract of Moringa oleifera seeds was obtained after drying in an oven; the obtained crude extract was dissolved in methanol and mixed with 10 times the amount of 80-100 mesh silica gel. -100 mesh), different polar components are separated, and 5 kinds of solvent elution of petroleum ether, chloroform, ethyl acetate, acetone and methanol are used successively; The acetone eluent is collected, and acetone is recovered under red...

Embodiment 2

[0026] like Figures 1 to 6 Shown, a method for testing the antiviral activity of the Moringa oleifera seed extract with anti-influenza virus activity, comprising the following steps:

[0027] Step 1. Select the test instrument, multi-function microplate reader: MK3 American ThermoFisher company. Inverted microscope: CKX53, Japan OLYMPUS company. Trinocular biological microscope: CX31 type, Japan OLYMPUS company. CO2 cell incubator: FORMA-311, ThermoFisher, USA. Ultra-clean bench: Heraguard ECO type, ThermoFisher, USA. Cryogenic centrifuge: Model S400-RT33D-E10FF, ThermoFisher, USA. Electronic balance: BP221S, produced by SARTORIUS in Germany. Micro vortex mixer: WH-2, produced by Shanghai Huxi Analytical Instrument Factory. High-speed microcentrifuge: LG15-W type, produced by Beijing Medical Centrifuge Factory. Low-speed centrifuge: DD-5M type, produced by Xiangyi Centrifuge Instrument Co., Ltd. Adjustable pipettes: 5, 10, 20, 500, 1000 μl types, manufactured by Therm...

Embodiment 3

[0049] like Figures 1 to 6 As shown, the test results:

[0050] 1. MoringaA has no obvious toxicity to Raw264.7 cells.

[0051]MoringaA with a gradient concentration of 1-512μM was used to co-incubate RAW264.7 cells for 24h. The results showed that with the increase of concentration, MoringaA exhibited inhibitory effect on RAW264.7 cells, and its CC50 and LogCC50 values ​​were 72.44μM and 1.86, respectively.

[0052] from image 3 It can be seen that compared with the normal group cells, the cell survival rate of the influenza virus infection group decreased significantly (P Figure 4 It can be seen that the cells in the influenza virus infection group showed obvious cytopathic effect and death, while the cytopathic effect of MoringaA treatment group was significantly alleviated, indicating that MoringaA has a good protective effect on host cells.

[0053] Second, MoringaA can significantly inhibit influenza virus.

[0054] from Figure 5 It can be seen that compared with ...

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Abstract

The invention discloses a moringa seed extract with anti-influenza virus activity, and belongs to the technical field of medical drugs, the moringa seed extract is named as Moringa A, and the chemical structure of the moringa seed extract is shown as follows: a specific test on the influence of the Moringa A on RAW264.7 macrophage proliferation shows that the moringa seed extract Moringa A with anti-influenza virus activity has no obvious toxicity on Raw264.7 cells, and the moringa seed extract Moringa A has no obvious toxicity on RAW264.7 macrophage proliferation. The Moringa A can be used for remarkably reducing the cytopathic effect caused by the influenza virus, and the Moringa A can be used for remarkably inhibiting the influenza virus; the MoringaA has a remarkable inhibition effect on the H1N1 type influenza virus; the MoringaA has a remarkable protection effect on host cells infected by H1N1 (Hepatocyte 1N1).

Description

technical field [0001] The invention relates to the technical field of medical drugs, in particular to a Moringa oleifera seed extract with anti-influenza virus activity. Background technique [0002] Moringa seeds are the seeds of Moringa oleifera, a plant of the Moringa genus (Moringa Adans) of the family Moringaceae, and have a wide range of edible and medicinal values. Moringa seeds are native to India and have been cultivated and eaten for nearly a thousand years. They are grown in Africa, tropical and subtropical regions. At the beginning of the last century, it was introduced from Myanmar to Mangshi, Yunnan Province, my country, and began to be planted on a large scale. In recent years, various provinces and regions south of the Yangtze River in my country have carried out a lot of planting. Domestic and foreign studies have shown that Moringa seeds are rich in nutrients such as oil, protein, and vitamins. In addition, they also contain natural active ingredients suc...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C07H15/203C07H1/08A61P31/16G01N30/02G01N30/06
CPCC07H15/203C07H1/08A61P31/16G01N30/02G01N30/06
Inventor熊永爱贺震旦
OwnerZUNYI MEDICAL UNIVERSITY