Extraction method of old medicine orange spice and application of old medicine orange spice in cigarette filter tip
A technology of extracting and extracting medicines, applied in essential oils/spices, tobacco, applications, etc., which can solve the problems of unseen old medicines and spices, obstacles to the application of old medicines and spices, and difficulty in extraction, so as to facilitate scale and industrialization Production, short cycle, and the effect of improving aroma quality
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Embodiment 1
[0036] A method for extracting old medicine orange spice, comprising the steps:
[0037] (1) Take 1kg of commercially available aged old medicinal oranges, add 5kg of purified water, and carry out two cell crushing ultrasonic extractions at room temperature, ultrasonic power 500W, and the first ultrasonic extraction time is 3h; the second ultrasonic extraction, extraction Time 2h; after the two extracts were combined, filtered with 500-mesh gauze, the obtained filtrate was refrigerated and settled at 4°C for 18h, and then filtered with a Buchner funnel to obtain the first extract of old medicine orange.
[0038] (2) The old medicine orange primary extract obtained in step (1) is separated by a 200-300nm microfiltration membrane module at room temperature, the solid content of the feed liquid is adjusted to 5.7%, and the microfiltration is carried out at 0.10MPa for 75min. The average flux is 17.21L / (m 2 h), collect the filtrate to obtain the old medicine orange microfiltratio...
Embodiment 2
[0047] The comparison test of membrane separation membrane concentration coupling technology and conventional separation process is carried out. The test of removal of inactive macromolecular impurities and the test of triacetin extraction effect are as follows:
[0048] When preparing Example 1 of the present invention, Comparative Example 1 and Comparative Example 2, the old medicine orange concentrate before extraction with triacetin was not added, respectively, using an AA3 continuous flow analyzer (German Bran Luebbe company) according to relevant standards: Determination refer to YC / T 216-2007 method, protein content determination refer to YC / T 249-2008 method, pectin content determination refer to YC / T 346-2010 method, cellulose content determination refer to YC / T 347-2010 method, to measure. The results are shown in the following table:
[0049] Table 1 Content comparison of macromolecular impurities in different processes
[0050]
[0051] Add 1:1 triacetin to th...
Embodiment 3
[0056] The old medicine orange flavor prepared in Example 1 is detected by gas chromatography-mass spectrometry coupled with GC-MS analysis method:
[0057] (1) Gas chromatography conditions: chromatography column: DB-5MS quartz capillary column (30m×0.25mm, 0.25μm); temperature program: the initial temperature is 60°C, maintained for 1min, and raised to 170°C at a rate of 8°C / min, Hold for 3min; rise to 260°C at a rate of 12°C / min, hold for 18min; inlet temperature: 200°C; oven temperature: 60°C; injection mode: split; column flow: 1mL / min; purge flow: 3mL / min; split ratio: 5:1; carrier gas: high-purity helium; injection volume: 1 μL; solvent delay: 2min.
[0058] (2) Mass spectrometry conditions: ionization mode: EI; electron energy: 70eV; interface temperature: 250°C; ion source temperature: 200°C; acquisition mode: scan; scanning range: 40-500amu.
[0059] (3) Qualitative analysis: By using mass spectrometry as a detector and gas chromatography, and using NIST spectral li...
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