Bacillus safensis T1-5 and application thereof

A technology of Bacillus suffolus and scab bacteria, applied in the direction of application, bacteria, and other chemical processes, can solve the problems of unsightly appearance of diseased potatoes, reduced quality and yield of damaged potato pieces, and small damage area, etc. The effect of pathogenic bacteria of scab, rich types of carbon sources, and wide application prospects

Pending Publication Date: 2022-08-02
SHIKEFENG CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, although the disease spots are limited to the cortex, the quality and yield of the damaged potato pieces can still be reduced, and they are not resistant to storage, and the appearance of the diseased potat

Method used

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  • Bacillus safensis T1-5 and application thereof
  • Bacillus safensis T1-5 and application thereof
  • Bacillus safensis T1-5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Isolation and identification of bacterial species

[0019] 1. Culture medium:

[0020] Potato Dextrose agar (PDA) medium (g / L): 200 g of potato, 20 g of glucose, 15 g of agar, 1000 mL of distilled water, natural pH.

[0021] 2. Strain source:

[0022] Bacillus safensis T1-5 was isolated from sugarcane field in Wuming District, Nanning City, Guangxi, China in November 2020.

[0023] Take 1g of soil sample and put it into a 50ml centrifuge tube, add 10ml of sterile water and shake evenly, shake at 37°C and 200rpm for 30min, and let it stand until it becomes clear to obtain a soil suspension.

[0024] The prepared soil suspension was serially diluted in the ultra-clean workbench, and 100 μL of 10 -3 , 10 -4 , 10 -5 The dilutions were spread on LB solid medium, repeated 3 times, and incubated at 37 °C for 24 h. After 24 hours, the colonies with different shapes were picked on the ultra-clean workbench and purified by streaking, cultured at 37°C for 24 hours, and the p...

Embodiment 2

[0031] Physiological and biochemical analysis of strains

[0032] The physiological and biochemical indicators of strain T1-5 were tested by Mérieux API 20NE non-fermentative G-bacillus identification kit. Bacillus safensis T1-5 can utilize pyruvate, glucose, maltose, Kohn gelatin, honey, arabinose and the like. The detailed results are as follows:

[0033] Table 1 Analysis of physiological and biochemical properties

[0034] Table1 Analysis of Physiological and Biochemical Properties

[0035]

Embodiment 3

[0037] Inhibitory effect of strain on Streptomyces scabies

[0038] Potato scab pathogen 4.1765 (purchased standard strain from CGMCC China General Microbial Culture Collection and Management Center, link for strain information: http: / / www.cgmcc.net / directory / detail?no=32374&number=4.176 5&genus=&species=&yiming= &page=1) Cultivated on potato medium at 28°C for 3 days; scrape spores and mycelium with 0.2ml sterile water to make a pathogen suspension of a certain concentration, and the mycelium can be grown when 100ul volume is required to coat the plate Spread over the flat surface.

[0039] Take the activated strain T1-5 in 50 ml of potato liquid medium at 37°C and cultivate it on a shaker for 2-5 days. After the fermentation broth was centrifuged at 12,000 r / min for 1 min, the supernatant was discarded, and the precipitate was suspended in the same volume of sterile water to prepare a cell suspension.

[0040] The test potato scab pathogen 4.1765 was spread on different pl...

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Abstract

The invention belongs to the technical field of potato scab prevention and treatment. The invention discloses a strain of bacillus safeensis T1-5, and the preservation number of the bacillus safeensis T1-5 is CGMCC (China General Microbiological Culture Collection Center) NO.23506. The invention further discloses a method for preparing the bacillus safeensis T1-5. The strain is preserved in the China General Microbiological Culture Collection Center on September 29, 2021, the preservation number is CGMCC No.23506, the preservation date is September 29, 2021, the preservation unit is called CGMCC for short, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus safensis T1-5 provided by the invention is simple to culture, short in period, free of ecological toxicity, resistant to saline and alkaline, rich in available carbon source variety, capable of effectively inhibiting pathogenic bacteria of the potato scab, and very wide in application prospect in biological prevention and control of the potato scab.

Description

technical field [0001] The invention belongs to the technical field of microbial control. Background technique [0002] Potato scab is a disease of tuber plants that damages the plant. It is manifested as nearly round to amorphous cork-like scab-like light brown lesions or plaques on the surface of the tuber, and the texture is rough to the touch. Generally divided into two symptoms, namely reticulated lesions and cracked lesions (easy to be mistaken for potato powder scab). Usually, although the diseased spots are limited to the cortex, the quality and yield of the infected potato pieces can still be reduced, and they are not resistant to storage, and the appearance of the diseased potatoes is unsightly, and the commodity grade is greatly reduced, which leads to certain economic losses. In the past, due to the low incidence rate and small damage area in our country, it did not attract people's attention. However, in recent years, the incidence area in Inner Mongolia, Heb...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14A01N63/22A01P1/00C12R1/07C09K101/00C09K109/00
CPCC12N1/20C09K17/14A01N63/22C09K2101/00C09K2109/00Y02A40/10
Inventor 仲乃琴巩俊花汪志琴刘月田赵盼解晓梅杨敬高俊岭曹晶晶朱红梅赵永龙刘伟
Owner SHIKEFENG CHEM IND
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