Microenvironment response type nano composite material for efficiently removing tumor cells as well as preparation method and application of nano composite material

A nanocomposite material and tumor cell technology, applied in the field of nanocomposite materials, can solve problems such as large side effects, insufficient therapeutic effect, poor drug enrichment effect, etc., and achieve low toxicity, poor drug resistance, and good biocompatibility Effect

Active Publication Date: 2022-08-05
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at problems such as low Fenton catalyst activity in current CDT therapy and poor drug enrichment effect, large side effects, and insufficient therapeutic effect in chemotherapy, and provides a microenvironment-responsive efficient tumor cell removal method that can be used in CDT-chemotherapy combined therapy Nanocomposite materials, their preparation methods and their applications

Method used

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  • Microenvironment response type nano composite material for efficiently removing tumor cells as well as preparation method and application of nano composite material
  • Microenvironment response type nano composite material for efficiently removing tumor cells as well as preparation method and application of nano composite material
  • Microenvironment response type nano composite material for efficiently removing tumor cells as well as preparation method and application of nano composite material

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preparation example Construction

[0043] The preparation of FeOCl can refer to: Journal of Hazardous Materials, 2019,

384:121494

[0044] The preparation of nanogel PAD can refer to the literature: Biomaterials Science, 2018, 7(1): 247-261 DOX can be purchased from Aladdin Reagent Company

[0045] BSA can be purchased from Sigma-Aldrich, the molecular weight is 66KDa

[0046] Human normal hepatocytes (L-O2) were purchased from Jiangsu KGI Biotechnology Company

[0047] Dulbecco's modified Eagle (DMEM) medium was purchased from Jiangsu KGI Biotechnology Company

[0048] Mouse breast cancer cells (4T1) were purchased from Jiangsu KGI Biotechnology Company

[0049] RPMI 1640 medium was purchased from Jiangsu KGI Biotechnology Company

Embodiment 1

[0050] Example 1 Preparation of PAD@FeOCl / DOX-BSA

[0051] 1. Preparation of Bulk FeOCl

[0052] Weigh 6g FeCl 3 ·6H 2 The O powder was placed in a crucible and heated in a microwave oven (700W) for 1 h. After cooling, the sintered product was taken out and fully ground into powder, then dispersed with acetone, sonicated for 15 min, and centrifuged at 8000 rpm for 5 min to remove unreacted FeCl in the supernatant. 3 , the precipitate was collected and redispersed with acetone. Centrifugation was repeated 3 times under the same conditions until the supernatant was colorless, and the final precipitated product was collected and placed in a vacuum drying oven overnight to obtain a massive reddish-brown material, which was fully ground to obtain FeOCl powder.

[0053] 2. Preparation of FeOCl NDs

[0054] Weigh 0.1 g FeOCl powder and 1.0 g NaCl powder into a dry ball mill jar. Set the running time of the ball mill to 12h, the rotational speed to 650rpm, and the interval betwe...

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Abstract

The invention provides a micro-environment response type nano composite material for efficiently removing tumor cells as well as a preparation method and application of the nano composite material. According to the nano composite material disclosed by the invention, iron oxychloride nano points (FeOCl NDs), doxorubicin (DOX) and bovine serum albumin (BSA) are loaded on polymer nanogel (PAD), so that the PAD-coated FeOCl / DOX-BSA nano composite material, which is called PFDB for short, is prepared. The material can be degraded under the action of excessive glutathione (GSH) in a tumor microenvironment, and FeOCl NDs and DOX are released. FeOCl NDs can catalyze H2O2 to generate hydroxyl radicals (OH) with strong oxidizing property to damage tumor cells; dOX can realize chemotherapy of tumors and synergistically enhance the treatment effect. The PFDB provided by the invention not only can effectively inhibit and remove tumor cells, but also has good colloidal stability and biological safety. In conclusion, the invention provides the tumor microenvironment response type nano composite material, and the efficient removal of tumor cells can be realized.

Description

technical field [0001] The invention relates to the technical field of nano-anti-tumor, in particular to a micro-environment responsive nano-composite material for efficiently removing tumor cells, a preparation method and application thereof. Background technique [0002] Cancer is still one of the most difficult diseases to overcome in the world. In 2020, there will be 19.3 million new cases of cancer diagnosed globally, and the number of cancer deaths has exceeded 10 million. With the development of modern medicine, scientists have developed a series of tumor treatment methods, including surgical resection, chemotherapy, radiation therapy, etc. Due to the complexity of tumor diseases, these conventional treatments are usually difficult to completely remove tumor cells and have certain side effects. Therefore, there is an urgent need to develop new efficient and low-toxicity tumor treatment methods. [0003] With the continuous development of nano-biomedicine, new anti-t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K33/26A61K47/32A61K47/42A61P35/00B82Y5/00B82Y30/00B82Y40/00C01G49/10A61K31/704
CPCA61K31/704A61K33/26A61K9/06A61K47/42A61K47/32A61P35/00B82Y5/00C01G49/10B82Y30/00B82Y40/00C01P2004/64A61K2300/00Y02A50/30
Inventor 宇文力辉肖航汪联辉李潇修尉峻
Owner NANJING UNIV OF POSTS & TELECOMM
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