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Application of morphine-dependent screening gene miR-124 and target protein IQGAP1 thereof

A miRNA-124 and target protein technology, applied in the field of morphine-dependent screening gene miR-124 and its target protein IQGAP1, can solve problems such as ignoring regulatory effects

Pending Publication Date: 2022-08-05
哈尔滨医科大学附属第一医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Screening for morphine dependence is mostly limited to a single gene or protein, while ignoring the regulatory effect between the two. The present invention provides the use of the morphine dependence screening gene miR-124 and its target protein IQGAP1

Method used

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  • Application of morphine-dependent screening gene miR-124 and target protein IQGAP1 thereof
  • Application of morphine-dependent screening gene miR-124 and target protein IQGAP1 thereof
  • Application of morphine-dependent screening gene miR-124 and target protein IQGAP1 thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] This embodiment uses QPCR technology to screen morphine dependencies and its target protein.

[0017] The specific screening method is: collecting morphine dependence on patients and health controls of 40 cases of blood specimens, according to the severity of opioids (OASI) to evaluate the severity of opioid addiction to patients, detect the morphine dependency group and the control group blood QPCR MIR-124 IQGAP1 level, the result is shown in Table 1.

[0018] Table 1 comparison with the population statistics and clinical variables of the control group of morphine dependencies

[0019]

[0020] Table 1 shows that the MiRNA-124 content (Z = -3.017, P <0.001), the MiRNA-124 content (Z = -3.017, P <0.001), the comparison of the population statistics in the control group (Z = -3.999, P < 0.001) Below the control group.

[0021] Analysis of the independent influencing factors of the total score of the OASI dependence of morphine, the results are shown in Table 2.

[0022] Tab...

Embodiment 2

[0026] This embodiment also uses biofluotosenase to react the combination of genes and target proteins.

[0027] In order to verify whether the MIR-214 directly targets IQGAP1, first of all, the PGL6-iQGAP1 carrier is constructed. IQGAP1 target gene is synthesized by ELK Biotechnology Company.

[0028] IQGAP1 is extended through PCR and connected to the overlapping sequence of the carrier, and then reorganized with the digestive carrier.

[0029] Related information is as follows:

[0030] IQAP15'-3'Primer Sequences:

[0031] 135iqgap1 F: CCGTGTGTAAGAGTCCCCCCAGAGAGACATCTCCCA

[0032] 136iqgap1 R: CTCTCGAGAGAGGGGGGGGGAAGTGTGTGACTTTTTC

[0033] 137mir-124Sequence: CCGuaaguggcgcacggaau

[0034] Expansion PCR through variability, annealing and extended programs. After 30 minutes of reorganization of 37 ° C, use high -efficiency DH5A to feel the state cells for transformation. After 1 day, a single cloning is picked for sequencing testing, and a single colonies with correct sequencing ...

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Abstract

The invention relates to application of a morphine-dependent screening gene miR-124 and a target protein IQGAP1 thereof, and belongs to the technical field of gene detection. Aiming at the problem that the screening of morphine dependence is mostly limited to a single gene or protein and the regulation effect between the morphine dependence and the single gene or protein is ignored, the invention provides the application of the morphine-dependent screening gene miR-124 and the target protein IQGAP1 of the morphine-dependent screening gene miR-124, namely the application of the morphine-dependent screening gene miR-124 and the target protein IQGAP1 of the morphine-dependent screening gene miR-124 in preparing a kit for screening morphine dependence. The morphine dependence screening gene miR-124 closely related to morphine dependence formation and the target protein IQGAP1 of the morphine dependence screening gene miR-124 are found, and an accurate basis is provided for diagnosis and severity judgment of morphine dependence in clinical or healthy screening. The target protein is further determined aiming at the screened differential gene, and the significance of the target protein in morphine dependence is defined.

Description

Technical field [0001] The invention is a genetic testing technology field, especially the use of morphine dependency screening gene MIR-124 and its target protein IQGAP1. Background technique [0002] In opioids, morphine is considered one of the most effective analgesic drugs. It is suitable for postoperative and cancer pain, but it is quite tricky to cause excessive use and addiction. Morphine and other opioids can induce extensive pharmacological activity. It occurs in the central nervous system and causes a series of symptoms, such as emotional disorders, can promote anxiety, depression and cognitive disorders. Frequent exposure to opioids can also lead to defects in learning, memory, attention, reasoning and impulse control. Morphine depends on patients with strong hiddenness, so the key to finding and positioning candidate genes is the key to the field of morphine dependencies. At present, the pathogenesis of morphine is not clear. Although there are studies on their genet...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6851C12N15/11
CPCC12Q1/6883C12Q1/6851C12Q2600/158C12Q2561/113C12Q2563/107C12Q2531/113
Inventor 胡建王晓红师晶晶胡晓蕊刘蕾张博程畅康传依赵娜王怀志卢佳朱爽白静波
Owner 哈尔滨医科大学附属第一医院