Rice flavamonocell hrf2 gene, recombinant vector and method for transgenic breeding plant therefor

A xanthomonas and gene technology, applied in botany equipment and methods, biochemical equipment and methods, using vectors to introduce foreign genetic material, etc., can solve the problem of unreported hrf2 gene application, etc., and achieve the effect of enhanced disease resistance

Inactive Publication Date: 2005-09-14
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, there is no report on the hrf2 gene and its application in plant transgenic breeding engineering tech...

Method used

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  • Rice flavamonocell hrf2 gene, recombinant vector and method for transgenic breeding plant therefor
  • Rice flavamonocell hrf2 gene, recombinant vector and method for transgenic breeding plant therefor

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Embodiment Construction

[0024] 1. The binary transformation of expressing hrf2 gene in plants—the construction of expression vector pBIH (pBI121::hrf2)

[0025] The plasmid used to construct the binary transformation-expression vector is transformed from T-DNA, except for the T-DNA 25bp repeat sequence (LB and RB), the promoter (P-nos) and terminator of the nopaline synthase gene In addition to T-nos, there are nptI used as a selection marker in prokaryotes (bacteria) and nptII used as a selection marker in eukaryotes (plants). The antibiotics used for selection are both Km and G- 418. Also in pBI121 are the promoter 35S (p35S) of the cauliflower mosaic virus expressed in eukaryotes and the uidA of the β-glucuronidase (Gus) gene. The hrf2 gene used as the target gene is inserted into the multi-enzyme cloning site between p35S and uid of the pBI121 plasmid (Fig. 3). The endonucleases used in the present invention are XbaI and BamHI. The binary vector pBI121 is a well-known public vector (Jefferson, ...

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Abstract

An hrf 2 gene of rice Xanthomonas is disclosed. It can be used as the target gene to be constructed on the Ti-plasmid dual-element vector pBI121 expressed in plant, so obtaining its recombinant vector pBI121:hrt2. Said recombinant vector is transferred to EHA105 to transform plant cells. The transformed regenerative plant (To generation) is screened in the culture medium and detected. The resultant transgenic plant has the improved broad-spectrum power to resist diseases and pests, and the improved other productive characteristics.

Description

(1) Technical field [0001] The invention "rice xanthomonad hrf2 gene, recombinant vector and method for plant transgenic breeding" belongs to the field of biotechnology and is specially used to improve plant disease resistance and other beneficial production traits through plant genetic engineering technology. (2) Technical background [0002] The gene hrf exists in two pathogenic varieties (pv.oryzae and pv.oryzicola) of rice Xanthomonas oryzae and other Xanthomonas (Xanthomonas spp.), which belongs to hypersensitive response and pathogenicity , hrp) gene cluster members. Most Gram-negative phytopathogenic bacteria have hrp gene clusters in which genes encoding proteins (Harpin) that induce allergic responses in plants vary. At present, the hrp gene clusters in four Gram-negative genera of plant pathogenic bacteria have been fully sequenced, and the four known hrp gene clusters have been classified according to the structure of the gene cluster, the composition of the oper...

Claims

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Application Information

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IPC IPC(8): A01H1/00C07H21/04C12N5/14C12N15/29C12N15/82
Inventor 王金生邵敏董汉松陈功友高学文李平杨春
Owner NANJING AGRICULTURAL UNIVERSITY
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