Blood ammonia reagent kit for enzyme detection

Abstract

The reagent kit for enzyme detection of blood ammonia consists of tromethamine in 50-150 mmol, Tween-20 in 0.1-2 ml, sodium azide om 0.5-2 g, lactose in 5-50 g, glutamate dehydrogenase in 500-1500 u, alpha-ketoglutaric acid in 2-20 mmol, sodium acetonate in 1-10 mmol, and reducing nicotin-amide-adenine dinuclectide phosphate in 0.1-0.5 mmol. After regulating pH to optimal value of 8.0, the components are dissolved, packed and freeze dried and may be cold stored for two years. After being re-dissolved, the components are stabilized at room temperature for 1 week and cold stored to stabilize for 1 month. The reagent kit has powerful interference resistance, medium hemolysis and chylemia, blood sugar below 20 mmol / L, bilirubin below 0.1 mmol / L, enzyme activity loss less than 5 %, MADPH loss less than 10 %, linearity reaching 0.2 mmol / L, CV inside batch of 3.8 % and CV between batches of 5.1 %, and recovering rate of 96.1 %.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for measuring blood ammonia by enzymatic method. Background technique [0002] There are diffusion method, ion exchange method, ion selective electrode method and enzymatic method for the determination of blood ammonia. The first three methods are difficult to carry out on a large scale in clinical practice due to the cumbersome determination, while the enzymatic method has the characteristics of high specificity, trace amount, rapidity and accuracy. However, the enzymatic method for measuring blood ammonia introduced in the current literature is only suitable for a small amount of scientific research, because the enzymes and coenzymes used in this method are only suitable for short-term use and have poor stability, making it difficult to use them in large quantities in various hospital laboratories for a long time. Contents of the invention [0003] The purpose of the present invention is to pro...

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Problems solved by technology

[0002] There are diffusion method, ion exchange method, ion selective electrode method and enzymatic method for the determination of blood ammonia. The first three methods are difficult to carry out on a large scale in clinical practice due to the cumbersome determination, while the enzymatic method has the characteristics of high specificity, trace amount, rapidity and accuracy. However, the enzymatic method for measuring blood ammonia introduced in the current literature is only suitable for a small amount of scientific research, because the enzymes and coenzymes used in this method are only suitable for short-term use and have poor stability, making it difficult to use them in large quantities in various hospital laboratories for a long time