A kind of production method of papaya enzyme special for beer

A production method, the technology of papain, is applied in the production field of special papain for beer, which can solve the problems such as the reduction of papain enzyme activity, affecting the application of papain, and being difficult to remove, so as to achieve fast extraction speed, easy filtration and separation, and enzyme production. Effect with little loss of vitality

Active Publication Date: 2021-11-09
GUANGXI NANNING TIANLV BIOLOGICAL PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the traditional papain production process, the enzyme is usually extracted by extraction or mechanical grinding. The production cycle of the process is long, and there are many impurities, which are difficult to remove. In the end, the enzyme activity of papain is greatly reduced, and the quality of the obtained enzyme is Poor, affecting the application of papain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of production method of papaya enzyme special for beer, comprises the following steps,

[0023] (1) Clean the fresh papaya, remove the skin and seeds, cut into pieces, mix with water, put it into a colloid mill and grind it evenly. The ratio of papaya to water is 1:1 to obtain papaya milk;

[0024] (2) Add compound enzyme in the papaya milk in the ratio of 0.8%, the compound enzyme is the combination of cellulase, hemicellulase, amylase, xylanase, pectinase, and its ratio is 18:11:13 :16:22, the enzyme activity is 2000U / mg, and then placed in a microwave extraction equipment for enzymolysis extraction, the microwave power is 600W, the frequency is 800MHz, the enzymolysis temperature is 45°C, and the enzymolysis time is 1.5h to obtain the enzymolysis solution ;

[0025] (3) After the enzymolysis is finished, the enzymolysis solution passes through a plate heat exchanger to rapidly reduce the temperature of the enzymolysis solution to 4°C, and then filter to obtai...

Embodiment 2

[0029] A kind of production method of papaya enzyme special for beer, comprises the following steps,

[0030] (1) Clean fresh papaya, peel and remove seeds, cut into pieces, mix with water, put it into a colloid mill and grind evenly, the ratio of papaya to water is 1:10, to obtain papaya milk;

[0031] (2) Add compound enzyme in the papaya milk in the ratio of 0.2%, the compound enzyme is the combination of cellulase, hemicellulase, amylase, xylanase, pectinase, and its ratio is 15:8:9 :14:16, the enzyme activity is 1500U / mg, and then placed in a microwave extraction equipment for enzymolysis extraction, the microwave power is 400W, the frequency is 500MHz, the enzymolysis temperature is 40°C, and the enzymolysis time is 0.5h to obtain the enzymolysis solution ;

[0032] (3) After the enzymolysis is completed, the enzymolysis solution passes through a plate heat exchanger to quickly reduce the temperature of the enzymolysis solution to 8°C, and then filters to obtain the cle...

Embodiment 3

[0036] A kind of production method of papaya enzyme special for beer, comprises the following steps,

[0037] (1) Clean fresh papaya, peel and remove seeds, cut into pieces, mix with water, put it into a colloid mill and grind evenly, the ratio of papaya to water is 7:10, to obtain papaya milk;

[0038] (2) Add compound enzyme in papaya milk in a ratio of 0.4%, compound enzyme is the combination of cellulase, hemicellulase, amylase, xylanase, pectinase, and its ratio is 17:11:11 :15:18, the enzyme activity is 1667U / mg, and then placed in a microwave extraction equipment for enzymolysis extraction, the microwave power is 500W, the frequency is 600MHz, the enzymolysis temperature is 42°C, and the enzymolysis time is 1.0h to obtain the enzymolysis solution ;

[0039] (3) After the enzymolysis is completed, the enzymolysis solution passes through a plate heat exchanger to rapidly reduce the temperature of the enzymolysis solution to 6°C, and then filter to obtain the clear liquid...

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PUM

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Abstract

The invention discloses a production method of special papaya enzyme for beer, which uses microwave enzymolysis to extract the enzyme, then quickly lowers the temperature of the enzymolysis solution through a plate heat exchanger, and finally performs microfiltration and filtration through a hollow fiber tube. Ultrafiltration to prepare a concentrated high-activity papain extract. The invention adopts the method of microwave enzymolysis to extract papain, adopts the combination of various enzymes for enzymolysis, the amount of enzyme is small, and the extraction speed is fast, and the cellulose fruit can be extracted after a short period of microwave enzymolysis. Decompose glue and other impurities, and separate the papain in it. The process cycle is short and the loss of enzyme activity is small. Compared with the traditional extraction method and mechanical wall-breaking extraction method, it is more efficient and faster.

Description

technical field [0001] The invention belongs to the technical field of papaya enzyme extraction, and in particular relates to a production method of special papain enzyme for beer. Background technique [0002] Papain is non-toxic and harmless, and is safe to use. Enzyme clear liquid enzyme with enzyme activity of 600,000 units / gram (determined by ultraviolet method) is generally used in beer. The enzyme has a strong ability to decompose protein, and can recombine hydrolyzate into proteinoid, which is unmatched by other enzymes. The application of papaya enzyme in the beer brewing process can improve the composition of saccharified wort, promote the degradation of fermented diacetyl, make the beer clear and antifreeze, and prevent the formation of beer turbidity in the cold storage process, and the effect is remarkable. [0003] In the traditional papain production process, the enzyme is usually extracted by extraction or mechanical grinding. The production cycle of the pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50
CPCC12N9/63C12Y304/22002
Inventor 卢福燊磨光庭
Owner GUANGXI NANNING TIANLV BIOLOGICAL PROD CO LTD
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