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Microbial pesticide and its prepn. method

A technology of microbial pesticide and preparation process, applied in the field of microbial pesticide and its production, can solve the problems of increasing pesticide residues, destroying ecological balance, rampant pests, etc., and achieving the effects of low cost and high spore production.

Inactive Publication Date: 2006-09-27
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the long-term, large-scale and repeated use of chemical pesticides, it has caused pollution to soil, water and the atmosphere; the increase in pesticide residues in agricultural by-products has also directly endangered human health and survival; at the same time, the resistance of pests and pathogens to pesticides has continued to deepen And expanding, at least 504 species of pests around the world have developed resistance to pesticides, and some pests have resistance up to tens of thousands of times; in addition, while chemical pesticides kill pests, they also kill natural enemies and other beneficial organisms, destroying Ecological balance, causing more rampant pests
In this way, the application amount and frequency of pesticides have to be increased, thus causing a vicious circle of chemical pesticide application

Method used

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  • Microbial pesticide and its prepn. method
  • Microbial pesticide and its prepn. method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one: the preparation of this pesticide preparation with spore content of 10 billion / ml

[0031] In a 70L fermenter, a 50L culture medium was formulated with 2.5% of soybean cake powder, 1.0% of sweet potato starch rust, 1.0% of glucose, 0.05% of magnesium sulfate, and 0.5% of calcium carbonate. Cool to 30°C after sterilizing by conventional methods, inoculate the production strains at a ratio of 5%, and cultivate for 30 hours at 30°C ± 1 aeration to obtain a fermentation broth with a spore content of 6.2 billion / ml. The fermented liquid is concentrated by sedimentation and then diluted to a suspension containing 10 billion spores / ml. Add 0.1% sodium benzoate as a preservative and 10% saponin as an emulsifier to obtain the product. The product has a spore content of more than 10 billion / ml, a suspension rate of 60-90%, a pH of 4.5-6.0, a fineness of 95% and a 100-mesh sieve, and a spore crystal ratio of 1:0.5.

Embodiment 2

[0032] Embodiment 2: Application test of using this microbial pesticide preparation to prevent and control eggplant Physalis ladybug

[0033] The test was carried out in a place in Ningbo, Zhejiang Province, using 200-fold dilutions of its 10 billion / ml preparation, the chemical pesticide control was 3000-fold dilutions of 50% phoxim emulsifiable concentrate, and a clear water blank control was set up, a total of 3 treatments. 15m per treatment area 2 , each repeated 4 times, arranged randomly. 7 days after spraying, investigate 10 points for each treatment, 10 plants for each point, count the population base, and calculate the control effect. The results are shown in Table 2. As can be seen from Table 2, the 10 billion / ml 200-fold dilution of this preparation has a field control effect of 60.0% on Physalis ladybug, which is similar to the effect of the 3000-fold dilution of the chemical pesticide phoxim EC.

[0034]

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Abstract

A microbial agricultural chemical for vegetables is prepared from the Bacillus thuringiensis subspecies Ba9808-2-3 through preparing culture meidum from carbon source (starch, dextrin, glucose, etc), nitrogen source (peptone, soybean cake powder, beef extract, etc), edible salt, phosphate, K salt and Ca salt, deep fermenting culture in liquid at 27-30 deg.C pH 7-9 for 25-40 hr, and post treatment. Its advantages are high effect and no environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and relates to a microbial pesticide and a production method thereof. Background technique [0002] Pests and diseases cause huge losses to agricultural production every year. In order to achieve stable and high yields, people have mainly used chemical pesticides to control them. Chemical pesticides have the advantages of high control efficiency, fast speed, broad insecticidal and antibacterial spectrum, low cost, and simple use, so they have been developed rapidly and become an important means of preventing and controlling pests and diseases to win agricultural production. According to statistics, the ratio of the chemical control area to the total control area in my country from 1985 to 2002 was as high as about 95%. Due to the long-term, large-scale and repeated use of chemical pesticides, it has caused pollution to soil, water and the atmosphere; the increase in pe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P7/00
Inventor 施跃峰竺利红李孝辉吴吉安桑金隆
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES