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Micropre filtering membrane near natural culturing method for enhancing microorganism cultivation property and its device

A technology of natural culture and microporous membrane, applied in the field of near-natural culture of microorganisms, methods and culture devices, can solve the problems of difficult active state and pure culture stage, and achieve the goal of enhancing culturability Effect

Inactive Publication Date: 2007-01-10
CHONGQING UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0008] (3) Potentially important ATP (which can reach 1 nM in seawater and freshwater and is likely to be derived from phytoplankton and other plankton) and other active substances for bacterial culture are difficult to provide in the pure culture stage and difficult to obtain Active state offers
All above-mentioned these methods, all do not possess the idea of ​​microorganism close to natural cultivation method of the present invention; Secondly, these devices either can not make cultured microorganisms communicate with the outside world, or are not suitable for microorganisms to be purely cultivated and form bacterium colonies on it, or do not have Form a complete general experimental method for pure culture of microorganisms

Method used

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  • Micropre filtering membrane near natural culturing method for enhancing microorganism cultivation property and its device
  • Micropre filtering membrane near natural culturing method for enhancing microorganism cultivation property and its device
  • Micropre filtering membrane near natural culturing method for enhancing microorganism cultivation property and its device

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Embodiment Construction

[0028] Next, the present invention will be described with reference to the accompanying drawings:

[0029] When microorganisms are cultured and isolated, the porous Petri dishes lined with microporous membranes (such as Figure 1A ) is packaged and sterilized, added with a sterile medium, and condensed for later use. Before culturing, first put the porous petri dish into the environment required by the microorganisms to be cultured, so that the liquid level of the external environment is higher than the surface of the medium, and the pressure difference promotes internal and external balance, so that the medium in the petri dish is the same as the active substance in the external environment Interpenetrate, balance, and remain consistent. After equilibrating for a certain period of time, inoculate microorganisms under sterile conditions or in other ways, and continue to return to the original environment for cultivation. At this time, the liquid level of the external environme...

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Abstract

An approximate natural culture method and equipment for culturing the microbes well includes arranging one or more layers of millipore filter membrane in a perforated culture container, sterilizing, adding aseptic culture medium, putting the container in the environment needed by the microbes to be cultured, inoculating the microbes in the culture medium, and culturing.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a method and a cultivation device for near-natural cultivation of microorganisms. Background technique [0002] The diversity of microorganisms in nature has attracted more and more attention from scientists from all over the world. According to the existing scientific evidence, scientists speculate that the number of microbial species should be within 10 5 -10 6 . Unfortunately, only less than 5,000 species of prokaryotes have been described in the literature, 3,500 species are recorded in the popular Approved List of Bacterial Names, and 3,100 species are recorded in the authoritative Bergey's Handbook. This means that known bacteria may only account for 1% or less of the bacterial diversity in the environment. Obviously, the 99% undescribed prokaryotic microorganisms must have more genetic, metabolic and functional diversity, an invisible natio...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12M3/00C12M3/04
Inventor 叶姜瑜
Owner CHONGQING UNIV
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