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High pressure environment marine microorganism enrichment culture and gravity separation device

A technology for enrichment and cultivation of marine microorganisms, which is applied in the field of enrichment and cultivation of marine microorganisms in high-pressure environments and gravity separation devices, can solve the problems of separation and cultivation of marine microorganisms, and cannot effectively improve the success rate of cultivation, and achieve the effect of improving cultivability

Active Publication Date: 2022-07-12
GUANGDONG LAB OF SOUTHERN OCEAN SCI & ENG GUANGZHOU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the solutions disclosed in the prior art can realize the enrichment and cultivation of microorganisms in the deep sea in situ, they do not separate and cultivate marine microorganisms, which cannot effectively improve the success rate of cultivation

Method used

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  • High pressure environment marine microorganism enrichment culture and gravity separation device
  • High pressure environment marine microorganism enrichment culture and gravity separation device
  • High pressure environment marine microorganism enrichment culture and gravity separation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] like figure 1 As shown, this scheme provides a high-pressure environment marine microorganism enrichment culture and gravity separation device, including enrichment and multi-level purification units: 1, gravity separation and culture unit 2, temperature control system 3, pressure control system 4 and a central Control system 5; the control end and the signal detection end of the enrichment and multi-level purification unit 1 and the gravitational separation and culture unit 2 are electrically connected to the central control system 5; the control ends of the temperature control system 3 and the pressure control system 4 are all centrally controlled The system 5 is electrically connected; wherein: the enrichment and multi-level purification unit 1 is used to realize the enrichment and multi-level purification process of marine microorganisms, obtain marine microorganism enriched bacteria liquid and inject the marine microorganism enriched bacteria liquid into the system....

Embodiment 2

[0079] More specifically, on the basis of Example 1, the high-pressure environment marine microorganism enrichment culture and separation technology involved in the device proposed in this scheme mainly includes two steps of enrichment and separation. First, after enrichment and cultivation in the enrichment and multi-level purification unit 1, the bacteria group with higher purity is obtained, and then enters the gravity separation and cultivation unit 2 under the condition of maintaining pressure for solid cultivation and separation, and through different medium and environmental conditions The combined process is simultaneously screened to obtain pure cultured strains. The realization principle is as follows:

[0080] Enrichment process: First, sterilize the microbial liquid enrichment culture chamber 11 and its accompanying pipe and valve parts, and then sequentially load the substrates to be cultivated such as deep-sea sediments, macroorganisms and extracts symbiotic with ...

Embodiment 3

[0084] preferably, as Figure 5 As shown, the separation and cultivation chambers 21 are sequentially provided with several, and the liquid injection unit 22 is connected to the central liquid injection pipe 212 of all the separation and cultivation chambers 21; the temperature control system 3 and the pressure control system 4 are respectively connected with all separation The culture chambers 21 are connected to construct a high-pressure and low-temperature environment consistent with the marine environment in each separation and culture chamber 21, so as to ensure that the enriched deep-sea microorganisms are separated and cultured under in-situ environmental conditions; It is connected with an independent environmental parameter detection unit 23 for real-time detection of temperature and pressure changes in each separation and culture chamber 21 , and transmits the detected data to the central control system 5 .

[0085] In the specific implementation, the microorganism s...

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Abstract

The invention proposes a high-pressure environment marine microorganism enrichment culture and gravity separation device, including an enrichment and multi-level purification unit and a gravity separation culture unit; under the construction of a high pressure and low temperature environment consistent with the marine environment, the enrichment and The multi-level purification unit is used to realize the enrichment and multi-level purification process of marine microorganisms, obtain the marine microorganism enriched bacterial liquid and inject the marine microorganism enriched bacterial liquid into the gravity separation and culture unit; the gravity separation and culture unit is used in the The high-pressure environment uses gravity to automatically streak, realizes the solid separation and cultivation of marine microorganisms, and effectively improves the cultivability of marine microorganisms. By reshaping the in-situ environment, the invention can carry out the enrichment culture and separation of marine microorganisms, so as to solve the problem of isolating and cultivating microorganisms in a high-pressure marine environment, effectively improve the cultivability of marine microorganisms, and provide an important basic means for developing and utilizing deep-sea microbial resources. .

Description

technical field [0001] The invention relates to the technical field of marine microorganisms, in particular to a high-pressure environment marine microorganism enrichment culture and gravity separation device. Background technique [0002] Marine microbial resources are important strategic resources in the world and have the most biodiversity. Techniques such as molecular ecology and metagenomics have significantly increased our knowledge of marine microbial diversity. However, currently 99% of marine microorganisms are not pure cultured, and many genes with unknown functions are not annotated or may be wrongly annotated in the database, so modern omics technology is not enough to provide enough information to understand all microorganisms, especially are microorganisms that have not yet been classified. To improve the culturability of microorganisms that have not yet been cultivated, innovative pure culture methods are an important way to explain the phenotype and genotyp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/26C12M1/04C12M1/02C12M1/00
CPCC12M33/22C12M41/40C12M41/12C12M47/12C12M41/48C12M23/38C12M23/58C12M27/02C12M29/20
Inventor 冯景春张偲杨志峰王屹
Owner GUANGDONG LAB OF SOUTHERN OCEAN SCI & ENG GUANGZHOU
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