High-pressure environment marine microorganism solid separation culture device and culture method
A technology for separation and culture of marine microorganisms, which is applied in the field of solid separation and cultivation devices for marine microorganisms in a high-pressure environment, can solve the problems of separation and cultivation of marine microorganisms, and cannot effectively improve the success rate of cultivation, and achieves the effect of improving cultivability.
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Embodiment 1
[0059] Such as figure 1 , figure 2 As shown, a high-pressure environment marine microorganism solid separation and cultivation device includes a high-pressure microorganism separation and cultivation chamber 1, a liquid injection unit 2, a temperature control system 3, a pressure control system 4, an environmental parameter detection unit 6 and a central control system 5; The high-pressure microorganism separation and cultivation chamber 1 is provided with a microorganism separation branch 12, which is used to separate the microorganisms and provide an area for microorganism cultivation to the greatest extent; The microorganism separation and cultivation chamber 1 is connected to the liquid injection unit 2 through the central liquid injection pipe 11; the deep-sea microorganism enrichment liquid is injected into the microorganism separation branch 12 of the high-pressure microorganism separation and cultivation chamber 1 from the liquid injection unit 2; the temperature cont...
Embodiment 2
[0076] More specifically, such as Figure 5 As shown, the high-pressure microorganism separation and cultivation chamber 1 is provided with several sequentially, and the liquid injection unit 2 is connected with the central liquid injection pipe 11 of all high-pressure microorganism separation and cultivation chambers 1; the temperature control system 3 and the pressure control system 4 It is connected to all high-pressure microorganism isolation and cultivation chambers 1 respectively, and is used to construct a high-pressure and low-temperature environment consistent with the marine environment in each high-pressure microorganism isolation and cultivation chamber 1, so as to ensure that the enriched deep-sea microorganisms are isolated and cultivated under in-situ environmental conditions Each high-pressure microorganism separation and cultivation chamber 1 is connected with an independent environmental parameter detection unit 6 for real-time detection of changes in temperat...
Embodiment 3
[0079] More specifically, on the basis of Example 1, this embodiment also proposes a method for solid separation and cultivation of marine microorganisms in a high-pressure environment, which is realized by applying a device for separation and cultivation of marine microorganisms in a high-pressure environment as claimed in the claim, such as Figure 6 shown, including the following steps:
[0080] S1: Sterilize all components of the marine microbial solid separation and cultivation device in a high-pressure environment to maintain a sterile state;
[0081] S2: Install the medium required for culture after filling the microorganism separation branch 12, and detect the tightness of the marine microorganism solid separation and culture device in a high-pressure environment;
[0082] S3: Turn on the temperature control system 3 to ensure that the temperature in the high-pressure microorganism separation and cultivation chamber 1 is consistent with the temperature conditions of th...
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