Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-pressure environment marine microorganism solid separation culture device and culture method

A technology for separation and culture of marine microorganisms, which is applied in the field of solid separation and cultivation devices for marine microorganisms in a high-pressure environment, can solve the problems of separation and cultivation of marine microorganisms, and cannot effectively improve the success rate of cultivation, and achieves the effect of improving cultivability.

Active Publication Date: 2022-05-10
GUANGDONG LAB OF SOUTHERN OCEAN SCI & ENG GUANGZHOU +1
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it can realize the enrichment and cultivation of microorganisms in the deep sea in situ, it does not separate and cultivate marine microorganisms, which cannot effectively improve the success rate of cultivation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-pressure environment marine microorganism solid separation culture device and culture method
  • High-pressure environment marine microorganism solid separation culture device and culture method
  • High-pressure environment marine microorganism solid separation culture device and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Such as figure 1 , figure 2 As shown, a high-pressure environment marine microorganism solid separation and cultivation device includes a high-pressure microorganism separation and cultivation chamber 1, a liquid injection unit 2, a temperature control system 3, a pressure control system 4, an environmental parameter detection unit 6 and a central control system 5; The high-pressure microorganism separation and cultivation chamber 1 is provided with a microorganism separation branch 12, which is used to separate the microorganisms and provide an area for microorganism cultivation to the greatest extent; The microorganism separation and cultivation chamber 1 is connected to the liquid injection unit 2 through the central liquid injection pipe 11; the deep-sea microorganism enrichment liquid is injected into the microorganism separation branch 12 of the high-pressure microorganism separation and cultivation chamber 1 from the liquid injection unit 2; the temperature cont...

Embodiment 2

[0076] More specifically, such as Figure 5 As shown, the high-pressure microorganism separation and cultivation chamber 1 is provided with several sequentially, and the liquid injection unit 2 is connected with the central liquid injection pipe 11 of all high-pressure microorganism separation and cultivation chambers 1; the temperature control system 3 and the pressure control system 4 It is connected to all high-pressure microorganism isolation and cultivation chambers 1 respectively, and is used to construct a high-pressure and low-temperature environment consistent with the marine environment in each high-pressure microorganism isolation and cultivation chamber 1, so as to ensure that the enriched deep-sea microorganisms are isolated and cultivated under in-situ environmental conditions Each high-pressure microorganism separation and cultivation chamber 1 is connected with an independent environmental parameter detection unit 6 for real-time detection of changes in temperat...

Embodiment 3

[0079] More specifically, on the basis of Example 1, this embodiment also proposes a method for solid separation and cultivation of marine microorganisms in a high-pressure environment, which is realized by applying a device for separation and cultivation of marine microorganisms in a high-pressure environment as claimed in the claim, such as Figure 6 shown, including the following steps:

[0080] S1: Sterilize all components of the marine microbial solid separation and cultivation device in a high-pressure environment to maintain a sterile state;

[0081] S2: Install the medium required for culture after filling the microorganism separation branch 12, and detect the tightness of the marine microorganism solid separation and culture device in a high-pressure environment;

[0082] S3: Turn on the temperature control system 3 to ensure that the temperature in the high-pressure microorganism separation and cultivation chamber 1 is consistent with the temperature conditions of th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a high-pressure environment marine microorganism solid separation culture device which comprises a high-pressure microorganism separation culture chamber, a liquid injection unit, a temperature control system and a pressure control system, the liquid injection unit injects deep-sea microorganism enrichment liquid into the high-pressure microorganism separation culture chamber, automatic streak separation operation is carried out on microorganisms in the high-pressure microorganism separation culture chamber, and the area is provided for microorganism culture to the maximum extent; the temperature control system and the pressure control system are used for constructing a high-pressure and low-temperature environment consistent with the marine environment in the high-pressure microorganism separation culture chamber, so that the enriched deep-sea microorganisms are separated and cultured under the in-situ environment condition. The invention further provides a high-pressure environment marine microorganism solid separation culture method, the in-situ marine temperature and pressure environment is constructed, automatic scribing is conducted in the high-pressure environment, solid separation and culture of microorganisms are achieved, and the culturability of the marine microorganisms is effectively improved.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation, in particular to a device for solid separation and cultivation of marine microorganisms in a high-pressure environment and a cultivation method. Background technique [0002] The ocean accounts for 71% of the earth's area, and marine microorganisms have a completely different ecological community structure and species diversity from terrestrial microorganisms. Deep-sea microbial resources are important strategic resources of the country, so they have also become a hot spot for development in various countries. The diversity of marine microorganisms determines its genetic diversity, metabolic diversity, chemical diversity and functional diversity. Marine microorganisms have formed unique and diverse adaptation mechanisms in the unique marine environment to cope with extreme environments such as high pressure, darkness, high salinity, high temperature, low temperature, and oligot...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/36C12M1/34C12M1/26C12M1/02C12M1/04C12M1/38C12M1/12C12M1/00C12N1/20C12N1/02
CPCC12M23/38C12M29/06C12M29/20C12M33/04C12M41/18C12M41/40C12M23/52C12M41/48C12M47/12C12M47/04C12M37/00C12M27/18C12N1/20C12N1/02
Inventor 冯景春张偲杨志峰王屹
Owner GUANGDONG LAB OF SOUTHERN OCEAN SCI & ENG GUANGZHOU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products