Biotechnological body bone tissue rack and its making process and use

A technology of tissue biology and products, applied in the direction of biochemical equipment and methods, tissue regeneration, microorganisms, etc., can solve the problems of limiting the practical application of scaffold products, not taking into account the different conditions of bone regeneration and angiogenesis, and affecting bone tissue regeneration

Inactive Publication Date: 2007-01-17
JIANGSU YENSSEN BIOTECH CO LTD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organic polymer polylactic acid (PLA for short), polyglycolic acid (PGA for short) or PLA and PGA compound (PLGA for short) (Hubel, Bio / Technology 1995 13 (6): 565-576; Thomson etc., Polymer scaffold processing in Principles in Tissue Engineering1997 p273-293; Cao et al., Plast Reconstr Surg.1997 100: 297-304; Minuth et al. Cell Tissue Research, 1998 291(1): 1-11; Weng Yulai et al., Shanghai Stomatology, June 2000 , Vol. 9 No. 2, pp. 94-96) also has a number of disadvantages: the acidic degradation products released during the polymer degradation process can cause inflammatory reactions and foreign body reactions in human tissues and affect bone tissue regeneration. At the same time, this Polymeroids do not have the biological activity of inducing human bone tissue regeneration
Therefore, the setting of scaffold products with uniform hole type in the prior art cannot take into account the different requirements of bone regeneration and angiogenesis, thus limiting the practical application of such scaffold products in bone tissue engineering

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biotechnological body bone tissue rack and its making process and use
  • Biotechnological body bone tissue rack and its making process and use
  • Biotechnological body bone tissue rack and its making process and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Silicon, calcium, and phosphorus microparticles have obvious biological induction effects on the proliferation of normal human osteoblasts, the biological activity of alkaline phosphatase, the synthesis and secretion of osteocalcin, and bone calcification

[0034] The human osteoblasts tested were obtained from healthy donors aged 20-25, and each group of cells was obtained from 0.2 cubic centimeters of superficial bone debris from one donor. A total of 5 groups of cells were used for the test. attached figure 1 The results shown are the mean and standard deviation of 5 sets of test data. Each 0.2 cm3 of superficial bone chips from donors can expand in the laboratory to 6 million to 10 million osteogenic cells. The cell culture solution for this test is pre-added with silicon, calcium and phosphorus inorganic raw material particles with a diameter of less than 10 microns at specific chemical concentrations or chemical concentration ratios shown below each ta...

Embodiment 2

[0035] Example 2: Silicon, calcium, phosphorus microparticles and organic polymer (PLGA) composite material has the performance of inducing normal human osteoblast proliferation and alkaline phosphatase biological activity better than a single organic polymer PLGA

[0036] This example bioassay demonstrates the induction of a set of nanocomposites of the present invention in in vitro cell culture, compared to a single organic polymer, PLGA, and to conventional polystyrene-diene cell culture dishes. The chemical atomic weight combination of the inorganic elements of the composite material is 67% of silicon, 22% of calcium, and 11% of phosphorus, and the volume ratio of the combination of inorganic elements and PLGA is 50:50. The composite material and the single organic polymer PLGA were made into discs with a diameter of 2 cm and a thickness of 1.5 mm by mold hot casting (200 degrees Celsius, 8 hours) (see Example 4 for detailed preparation steps). The discs were placed in rou...

Embodiment 3

[0037] Example 3 Diffusion and distribution of silicon ions after implantation of silicon nanomaterials in animal models, and ion distribution in induced new bone tissue

[0038] This biological test uses adult white rabbits as the animal model. A bone cavity with a diameter of 0.5 cm was prepared in the fibula of the animal with a conical drill, and then filled with 50-80 nanometer silicon-calcium-phosphorous (atomic weight percentage 67:22:11) composite material particles, and the wound was sutured. When the experimental animals were fed to 2 and 8 weeks respectively, the material filling site and surrounding tissues were taken out by the second operation, fixed in 10% formalin, embedded in resin, cut 1 mm slices from the longitudinal direction, and then placed in a radioactive ion analyzer Measure the ion concentration facets on both sides of the interface between the material filling area and the surrounding animal body tissue. The data in Table 1 below are the mean value...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The present invention discloses a microgranule composite material scaffold product with human bone tissue regeneration induction activity and its use. Said scaffold product uses the silicone, calcium and phosphorus microgranule combination capable of actively inducing bone cell of human body to reproduce and make differentiation and forming new bone and calcifying as biological active substance and uses organic polymer as carrier, and has the three-dimensional structure meeting differnt biological characteristics of bone regeneration and vessel regeneration and external anatomical form, so that it can be effectively used for regeneration and renovation of bone tissue defect and bone plastic therapy of human body. Said invention also discloses the preparation method of said scaffold.

Description

field of invention [0001] The present invention relates to a composite material product as a human bone tissue bioengineering scaffold product, and more specifically relates to a novel medical microparticle composite material product as a human bone tissue bioengineering scaffold product with human bone tissue regeneration inducing activity, and Preparation method and application in bone tissue bioengineering. Background of the invention [0002] Human bone tissue bioengineering is a process in which resorbable biomaterials are used as scaffolds to guide autologous bone regeneration. The physical and chemical properties and three-dimensional structure of biomaterial scaffold products are the key to directly affect the regeneration of bone tissue. Based on the standards of molecular compatibility of biomaterials, the materials used for human transplantation devices or biological tissue engineering scaffold products must not only be safe, but also have the ability to induce r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/28A61L27/56A61L27/54A61L27/00A61F2/00A61F2/30A61L27/44A61L27/50C12N5/08
CPCA61F2230/0082A61F2002/30957A61F2002/30242A61F2/30942A61F2230/0069A61F2310/00293A61F2002/30261A61F2/3094A61F2/3099A61L2430/02A61L27/44A61F2/28A61F2002/30199A61F2002/30224A61F2230/0063A61F2230/0071A61L27/56A61P19/00
Inventor 周来生
Owner JIANGSU YENSSEN BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap