Method for producing glucolipid substance using ganglioside as main component

A technology of gangliosides and production methods, applied in the field of health care products and pharmaceuticals, can solve problems such as complex processes, achieve high yield, enhance learning and memory, and promote the recovery of nerve function and behavior

Inactive Publication Date: 2007-02-21
上海脑力键生物医学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method uses two organic solvents for extraction, and the process is relatively complicated. Petroleum ether is a volatile, flammable, and explosive liquid, which has high environmental requirements for industrial production. Therefore, a simpler and safer production method is used. Craftsmanship is a matter of great concern in the industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 1Kg of fresh pig brain, remove sundries, rinse off blood stains and drain, add 4Kg of 75% edible alcohol, stir with a homogenizer for 3 hours at a speed of 3000 rpm and room temperature, filter, and concentrate the filtrate in a vacuum. A product is obtained, and the product contains a glycolipid substance of ganglioside ≥ 90%.

Embodiment 2

[0022] With 1Kg of frozen bovine brain, remove sundries, rinse off blood stains, drain, add 1Kg of deionized water, 1Kg of 95% edible alcohol, mash it with a homogenizer for 3 minutes at a rotating speed of 3000 rpm and room temperature, and then Add 3Kg of 85% edible alcohol, stir for 2 hours (at a temperature of 30° C.), filter, and concentrate the filtrate in vacuo to obtain a product containing ≥90% of the glycolipid substances of gangliosides.

Embodiment 3

[0024] Take 1Kg of frozen pig brain, remove sundries, rinse off blood stains, drain, add 2Kg of 75% edible alcohol, mash it with a homogenizer at a speed of 3000 rpm and room temperature for 5 minutes, then add 90% edible alcohol 3Kg, stirred for 1 hour (temperature 50°C), filtered, added 2Kg of 95% alcohol to the supernatant, extracted at room temperature for 2 hours, the filtrate was concentrated in vacuo, and the product was obtained, and the product contained ganglioside glycolipids ≥ 90% .

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Abstract

The present invention relates to a method for producing glycolipides substance using ganglioside as main component, sand method includes the following steps: pulverizing animal brain tissue, adding 20-100% alcohol solvent to make extraction, in which the ratio of dose of alcohol and weight of brain tissue is 1-10:1, filtering or centrifugalizing, concentrating filtrate, the concentrate and its freeze-dried powder can be diluted with water or mixed with edible auxiliary material, and can be made into the oral brain health-care product with various forms.

Description

technical field [0001] The invention relates to the fields of health care products and medicines, and more specifically relates to a production method for extracting glycolipid substances mainly gangliosides from brain tissue. Background technique [0002] Gangliosides are a class of membrane glycolipids containing N-acetylneuraminic acid, which exist in the membranes of mammalian cells, especially neurons, and are natural components of neuron membranes. A large number of clinical studies have proved that exogenous ganglioside has the characteristics of fat solubility, and it can effectively exert its biological activity after being embedded in the nerve cell membrane. Regarding the method for extracting gangliosides, it has been reported as early as the five or six ages that Sigma (SIGMA) company in the U.S. extracted from bovine brain with chloroform-methanol, and it was also reported that chloroform-ethanol solution was used to extract (CN931125788.2 ), but all adopt a l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/10C07H15/04A61K31/7032A61P25/00A23L1/30A23L33/10
Inventor 高翔战国利胡家庆王毅武卞正云
Owner 上海脑力键生物医学有限公司
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