Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Methods of producing target molecule in transgenic animal and purification of target molecule

A transgenic animal and transgenic technology, applied in biochemical equipment and methods, peptide preparation methods, chemical instruments and methods, etc.

Inactive Publication Date: 2002-12-25
GTC BIOTHERAPEUTICS INC
View PDF14 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The target molecule is preferably a target polypeptide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods of producing target molecule in transgenic animal and purification of target molecule
  • Methods of producing target molecule in transgenic animal and purification of target molecule
  • Methods of producing target molecule in transgenic animal and purification of target molecule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0081] The present invention relates to the transgenic expression and purification of polypeptides from biological systems. More specifically, the invention relates to transgenic systems for expressing and purifying polypeptides, and methods of using these systems. The patents and publications cited herein reflect the prior knowledge of those skilled in the art and are hereby incorporated by reference in their entirety.

[0082] The present invention provides systems and methods for purifying target molecules present in biological systems. Systems and methods according to the invention utilize existing multivalent binding polypeptides, such as transgenically produced multivalent binding polypeptides, as affinity media to purify these target molecules. Preferably, the multivalent binding polypeptide binds both the target molecule and a matrix, such as a matrix capable of phase separation. These systems and methods provide many advantages over existing systems. The systems an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides systems and methods for preparing and purifying target molecules present in biological systems. The systems and methods according to the present invention utilize a transgenically expressed multivalent binding polypeptide as an affinity medium for purifying the target molecule to purify the target molecule. The transgene multivalent binding polypeptide not only binds to the target molecule (for example, the bindable epitope of the target molecule), but also binds to the substrate.

Description

Background of the invention [0001] Preparation and purification of polypeptides for therapeutic and diagnostic purposes has become an important industry in recent years. Early attempts at recombinant polypeptide expression were performed in bacteria using plasmid expression vectors. For example, US Patent 4,816,397 discloses recombinant expression of immunoglobulins in bacteria. These methods are limited by the need to open or lyse the bacteria to obtain the recombinant polypeptide as part of a complex mixture containing bacterial proteins and other bacterial components. Yet another limitation is caused by the non-native oxidation state of recombinant polypeptides in bacteria, which leads to incorrect polypeptide folding. [0002] Some of these problems are alleviated by recombinant expression of polypeptides in mammalian cell culture fermentation systems. US Patent 5,639,640 discloses the recombinant expression of human follicle stimulating hormone (FSH) in mammalian cell ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027C07K1/14C07K1/22C07K14/47C12N15/09C12N15/85
CPCA01K2207/15A01K2217/00A01K2217/05A01K2227/10A01K2267/01C07K1/22C12N15/8509C12N2830/008A01K67/00
Inventor H·米德S·P·富尔顿Y·埃克拉德
Owner GTC BIOTHERAPEUTICS INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com