Grassland locoism toxin vaccine

A vaccine and locust technology, applied in the direction of antitoxins, peptide/protein components, antibody medical components, etc., can solve problems such as drug residues, grassland desertification, and destruction of grassland ecosystems

Inactive Publication Date: 2003-02-12
杨凌大农生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current methods of manual excavation and chemical control will further desertify the grassland and destroy the...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: according to the technical scheme of the present invention, prepare prairie locotoxin vaccine, carry out according to the following steps:

[0025] The first step, the synthesis of SW-active ester (quaternary ammonium salt):

[0026] Reaction formula

[0027] The operating procedure is: after dissolving 17.3mg (0.1mmol) of SW completely in dry acetone solution, add 23.6mg (0.1mmol) of active ester prepared with bromoacetic acid, stir and reflux at 80°C for 9h, filter, wash with acetone, five Phosphorus oxide was vacuum dried. Calculate the yield. MP, IR, elemental analysis and identification.

[0028] The second step, the synthesis of SW-BSA:

[0029] Reaction formula

[0030] The operating procedure is: take 167.5mg (0.0025mmol) of crystalline BSA, dissolve it completely in 4mL of distilled water, add 40.9mg (0.1mmol) of the prepared quaternary ammonium salt, and stir in an ice bath for 18h. After measuring the binding rate, the reaction product...

Embodiment 2

[0034] Embodiment 2: according to the technical scheme of the present invention, prepare prairie locotoxin vaccine, carry out according to the following steps:

[0035] The first step, the synthesis of SW-active ester (quaternary ammonium salt):

[0036] Reaction formula

[0037] The operating procedure is: take 34.6mg (0.2mmol) of SW completely dissolved in dry acetone solution, add 47.2mg (0.1mmol) of active ester prepared with bromoacetic acid, stir and reflux at 75°C for 9.5h, filter, wash with acetone, Phosphorus pentoxide was vacuum dried. Calculate the yield. MP, IR, elemental analysis and identification.

[0038] The second step, the synthesis of SW-BSA:

[0039] Reaction formula

[0040] The operating procedure is: take 335mg (0.005mmol) of crystalline BSA, dissolve it completely in 6mL of distilled water, add 81.8mg (0.2mmol) of the prepared quaternary ammonium salt, and stir in an ice bath for 18.5h. After measuring the binding rate, the reaction product w...

Embodiment 3

[0044] Embodiment 3: according to the technical scheme of the present invention, prepare prairie locotoxin vaccine, carry out according to the following steps:

[0045] The first step, the synthesis of SW-active ester (quaternary ammonium salt):

[0046] Reaction formula

[0047]The operating procedure is: take 86.5mg (0.5mmol) of SW and dissolve it completely in dry acetone solution, add 118mg (0.5mmol) of active ester prepared with bromoacetic acid, stir and reflux at 85°C for 8.5h, filter, wash with acetone, five Phosphorus oxide was vacuum dried. Calculate the yield. MP, IR, elemental analysis and identification.

[0048] The second step, the synthesis of SW-BSA:

[0049] Reaction formula

[0050] The operating procedure is: take 837.5mg (0.0125mmol) of crystalline BSA, dissolve it completely in 16mL of distilled water, add 204.5mg (0.5mmol) of the prepared quaternary ammonium salt, and stir in an ice bath for 17.5h. After measuring the binding rate, the reaction...

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PUM

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Abstract

A grassland locoism toxin vaccine for animals raised on grassland is prepared through firstly reaction of bromoacetic acid on N-hydroxysuccinimide to obtain active ester, further, synthesis with swainsonic to obtain N-carboxymethyl swainsoniue, finally condensation with BSA to obtain SW-BSA, then dialysis, freeze-drying and copmletely emulsifying with Folin's assistant to obtain SW vaccine. Its advantages are long active period (12 months), high protextion rate up to 85% or more, and full use of loco weed resource.

Description

[0001] 1. Field [0002] The invention belongs to a biochemically synthesized plant toxin vaccine, relates to the technical fields of toxicology, organic synthesis and immunology, in particular to a prairie locoweed toxin vaccine. 2. Background technology [0003] Locoweed is the general term for poisonous plants of the genus Astragalus and Oxytropis in the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast pastoral areas of Northwest, North China, and Southwest China, and the endangered area reaches 4 million hectares. Locoweed contains the main toxic ingredient indozidine alkaloid - swainsonine (SW), which can cause poisoning and death of a large number of animals, and more seriously cause abortion, infertility, fetal malformation and male infertility in female animals. cause huge economic losses. Although some progress has been made at home an...

Claims

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Application Information

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IPC IPC(8): A61K38/38A61K39/00A61P39/02
Inventor 童德文曹光荣赵宝玉葛鹏斌
Owner 杨凌大农生物技术有限公司
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