Reagent box for fast detecting methyl amphetamine and its preparation process

A technology of methamphetamine and preparation process, which is applied in the directions of biological testing, measuring devices, material inspection products, etc., can solve the problems of long time, difficult monitoring work, and high cost of detecting drugs, and achieves the effect of strong specificity

Inactive Publication Date: 2003-04-16
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatographic and immunological methods are costly, inconvenient, and time-consuming to detect drugs, especially for front-line public security personnel in drug inspection and anti-drug and monitoring work of anti-drug rehabilitation institutions in small cities.
There are relevant similar detection method bibliographical reports both at home and abroad (U.S. Patent 5,026,827. Use the conjugate of N-(4-aminobutyl) amphetamine and protein to produce the antibody that can combine with methamphetamine; Kur

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] a) The temperature is at 15°C;

[0066] b) vacuum drying time of 5 hours;

[0067] c) Atmospheric pressure in the vacuum container when dry 0.05 millibar (mba). 5. The packaging and use of the detection kit

[0068] The use of the plastic box of the present invention makes the operation process of detection more convenient:

[0069] i. Use a straw to take about 0.2ml of urine sample, and then drop it all into the sample hole.

[0070] ii. Read the result 5 minutes after adding the sample.

[0071] iii. There are two color bands in the observation window, indicating that there is no drug in the sample, which is a negative result; only the color band in the C line area in the observation window indicates that there is drug in the sample, which is a positive result.

Embodiment 2

[0074] a) The temperature is at 25°C;

[0075] b) Vacuum drying time is 2 hours;

[0076] c) Atmospheric pressure in the vacuum container when dry 0.01 millibar (mba). 5. Packaging and use of the test kit

[0077] The use of the plastic box of the present invention makes the operation process of detection more convenient:

[0078] i. Use a straw to take about 0.2ml of urine sample, and then drop it all into the sample hole.

[0079] ii. Read the result 5 minutes after adding the sample.

[0080] iii. There are two color bands in the observation window, indicating that there is no drug in the sample, which is a negative result; only the color band in the C line area in the observation window indicates that there is drug in the sample, which is a positive result.

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PUM

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Abstract

The detection test paper strip includes four parts: the filtering sample paper, glass fiber of antibody of amphetamine methyl marked by colloidal gold, in T line area (detection area), coated by amphetamine methyl-BSA and in C line area (quality control area), film of pyroxylic coated by ovine antibody for anti the antibody of rat, absorbent paper. The formula of the process solution of the filtering sample paper is as follows. Brij 0.05-3 wt%, Triton X-100 0.01-2%, BSA 0.5-3%, NaCI 3-9%, pure water 90-97%, PB buffer solution 0.01-0.03 mmol. The invention provides the features of high sensitivity, strong specificity, good repeatability, fast and accurate.

Description

1. Technical field: [0001] The invention relates to a detection kit made by using anti-methamphetamine monoclonal antibody and colloidal gold immunochromatography technology, and a method for detecting methamphetamine in human urine by using the kit. It belongs to the field of medical testing supplies. 2. Background technology: [0002] Methamphetamine (Methamphetamine, also known as methamphetamine, methamphetamine, commonly known as "ice") is a therapeutic drug that mimics sympathetic stimulation. It belongs to the central nervous system stimulant and is also a psychotropic drug strictly controlled by the world and my country. It is an α and β receptor stimulant. Acute administration of higher concentrations will lead to increased excitement of the central nervous system, resulting in abnormal euphoria, increased sensitivity, faster heartbeat and breathing, and perceived energy. Long-term or heavy use will produce tolerance and physical dependence, and lead to abuse. Fu...

Claims

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Application Information

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IPC IPC(8): C12P21/08G01N33/53G01N33/531G01N33/543G01N33/558G01N33/577G01N33/94
Inventor 刘剑
Owner SHANGHAI CHEMTRON BIOTECH
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