Method of detecting dust mite allergen

Abstract

A process for detecting the content of main allergen Der f2 for dust mite features that the capillary electrophoresis and electrochemical detection technique with long fused quartz capillary tube andthree-electrode system in dectecting pool is used. Its advantages are high effect and high speed.

Description

technical field

[0001] The invention belongs to the field of chemical testing, and in particular relates to a method for detecting the main allergen of dust mite, especially a capillary electrophoresis electrochemical detection technology for Der f2, the main allergen of dust mite. Background technique

[0002] Dust mite is a strong allergen that causes various allergic diseases such as exogenous asthma, allergic dermatitis and infantile eczema. Immunotherapy is different from drug treatment that only improves symptoms. It is based on changing the patient's autoimmune reactivity to achieve clinical cure.

[0003] Specific immunotherapy mainly uses specific allergens for desensitization (or desensitization) treatment to regulate the immune function of the body. At present, in domestic clinics, the infusion of dust mite allergens is mostly used to make injections to treat chronic allergies. But they are crude products, and the content of main allergens cannot be measured. I...

Examples

Embodiment

[0014] 1. Preparation of laboratory standard product Der f2 Take the Dermatophagoides farinae culture medium and degrease it with acetone, immerse it in physiological saline at 1:5 (w / v), stir at 4°C for 48 hours, centrifuge at low temperature (10000rpm) for 20 minutes, and take the supernatant liquid. The supernatant was subjected to Sephadex G-200 column chromatography, eluted with 0.01mol / L PB, pH 7.2, and used as a skin prick test for mite asthmatic patients to collect the eluted fraction with biological activity. Partially in the "valley" between two eluting peaks. The biologically active fraction eluted by Sephadex G-200 chromatography was subjected to DEAE-cellulose chromatography, and the protein peak was collected after elution with 0.01mol / L PBS pH 7.2, and the collected protein peak was concentrated Perform zone electrophoresis. After electrophoresis, the gel bands are cut into strips, respectively soaked in double distilled water, and placed at 4°C for 4 days to c...

technical field

[0001] The invention belongs to the field of chemical testing, and in particular relates to a method for detecting the main allergen of dust mite, especially a capillary electrophoresis electrochemical detection technology for Der f2, the main allergen of dust mite. Background technique

[0002] Dust mite is a strong allergen that causes various allergic diseases such as exogenous asthma, allergic dermatitis and infantile eczema. Immunotherapy is different from drug treatment that only improves symptoms. It is based on changing the patient's autoimmune reactivity to achieve clinical cure.

[0003] Specific immunotherapy mainly uses specific allergens for desensitization (or desensitization) treatment to regulate the immune function of the body. At present, in domestic clinics, the infusion of dust mite allergens is mostly used to make injections to treat chronic allergies. But they are crude products, and the content of main allergens cannot be measured. I...

Examples

Embodiment

[0014] 1. Preparation of laboratory standard product Der f2 Take the Dermatophagoides farinae culture medium and degrease it with acetone, immerse it in physiological saline at 1:5 (w / v), stir at 4°C for 48 hours, centrifuge at low temperature (10000rpm) for 20 minutes, and take the supernatant liquid. The supernatant was subjected to Sephadex G-200 column chromatography, eluted with 0.01mol / L PB, pH 7.2, and used as a skin prick test for mite asthmatic patients to collect the eluted fraction with biological activity. Partially in the "valley" between two eluting peaks. The biologically active fraction eluted by Sephadex G-200 chromatography was subjected to DEAE-cellulose chromatography, and the protein peak was collected after elution with 0.01mol / L PBS pH 7.2, and the collected protein peak was concentrated Perform zone electrophoresis. After electrophoresis, the gel bands are cut into strips, respectively soaked in double distilled water, and placed at 4°C for 4 days to c...

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