Method for expressing thymopolypeptides šŒ1 by use of escherichia coli and its application

Abstract

A process for expressing thymopeptide alpha 1 by colibacillus includes PCR amplification to obtain the full-length gene of thymopeptide alpha 1, endonuclease severing at both ends, and cloning to colibacillus for stable expression. After purified, it can be used as injection to treat immunodeficiency diseases.

Description

technical field

[0001] The invention belongs to biotechnology and relates to genetic engineering, especially a method for expressing thymosin α1 in Escherichia coli, which can be used for adjuvant treatment of diseases with low immune function. Background technique

[0002] The thymus is an important immune organ in the body and plays an important role in the development of the lymphatic system and maintaining the normal function of the immune system. Especially in anti-infection, anti-tumor, transplantation and autoimmune diseases, it has special significance. As the body ages, the thymus gradually shrinks and degenerates, and the incidence of tumors, infections, and immune diseases gradually increases. A large number of studies have shown that the calf thymus extract (containing thymosin) can be used as an adjuvant treatment for tumors, infections and other diseases caused by hypofunction of the thymus. However, since the thymus extract of animals is a mixed component, t...