Method and device for quick testing beta-excitant by chromatograph

A detection method and technology for stimulants, applied in the field of rapid detection of β-stimulant gold standard chromatography, can solve the problems of complex detection methods of β-stimulant content

Inactive Publication Date: 2003-08-13
王旻子
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection method of β-stimulant content in food is relatively complicated, usually using HPLC or GC / MS, etc.

Method used

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  • Method and device for quick testing beta-excitant by chromatograph
  • Method and device for quick testing beta-excitant by chromatograph

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Make gold-labeled antibody with 70nm colloidal gold particles (purple) and 100ug / ml clenbuterol antibody solution; And it is coated on one end of polyester film with the amount of 2ul / cm; Renbuterol antibodies are polyclonal antibodies.

[0016] The clenbuterol-BSA conjugate 2000ug / ml (PBS solution) was coated on the nitrocellulose membrane in the amount of 1ul / cm, located on the upper end of the gold-labeled antibody, forming a T-line area;

[0017] Coat the upper end of the nitrocellulose membrane conjugate region with 4000ug / ml of anti-antibody (PBS solution) at an amount of 1ul / cm to form a C-line region.

[0018] Drying is required after coating.

[0019] The polyester film can be fixed on the substrate by common pasting methods.

[0020] At the same time, one end of the polyester membrane gold-labeled antibody was connected to a fixed glass fiber, and the other end of the polyester membrane, that is, the upper end of the C-line area, was connected to filter pape...

Embodiment 2

[0027] The difference from Example 1 is that this example uses a monoclonal antibody, which is very convenient when detecting clenbuterol, clenbuterol.

[0028] The product can be used as a means of rapid on-site results for the primary screening of pigs, cattle, sheep and other livestock for the use of β-stimulants. Urine, milk, blood, muscle or visceral extracts can all be used as samples.

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Abstract

A gold-labeling chromatography method for quickly detecting beta-excitant includes reaction of liquid speciment with gold-labelled clenbuterol antibody to obtain antigen-antibody compound, competing with clenbuterol-BSA coupled substance for the compound antibody, and reaction of said antigen-antibody compound or gold labeling antibody with the antibody. Its advantage is high speed.

Description

technical field [0001] The invention relates to a rapid detection method and device of β-doping agent gold standard chromatography Background technique [0002] Ordinary β-stimulants, if taken in excess, will cause great harm to the human body, such as carcinogenicity and poisoning. The state expressly prohibits its use in related feed. [0003] At present, the detection methods for β-stimulant content in food are relatively complicated, and HPLC or GC / MS are usually used. Contents of the invention [0004] A kind of β-doping agent gold standard chromatography rapid detection method comprises the following steps: [0005] (1) Take the liquid sample and react with the gold-labeled clenbuterol antibody to form an antigen-antibody conjugate; [0006] (2) Combine with clenbuterol-BSA conjugate again; [0007] (3) Finally, the antigen-antibody conjugate reacts with the anti-antibody. [0008] The above-mentioned...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/543G01N33/558
Inventor 王旻子
Owner 王旻子
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