Method and device for quick testing beta-excitant by chromatograph
A detection method and technology for stimulants, applied in the field of rapid detection of β-stimulant gold standard chromatography, can solve the problems of complex detection methods of β-stimulant content
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Embodiment 1
[0015] Make gold-labeled antibody with 70nm colloidal gold particles (purple) and 100ug / ml clenbuterol antibody solution; And it is coated on one end of polyester film with the amount of 2ul / cm; Renbuterol antibodies are polyclonal antibodies.
[0016] The clenbuterol-BSA conjugate 2000ug / ml (PBS solution) was coated on the nitrocellulose membrane in the amount of 1ul / cm, located on the upper end of the gold-labeled antibody, forming a T-line area;
[0017] Coat the upper end of the nitrocellulose membrane conjugate region with 4000ug / ml of anti-antibody (PBS solution) at an amount of 1ul / cm to form a C-line region.
[0018] Drying is required after coating.
[0019] The polyester film can be fixed on the substrate by common pasting methods.
[0020] At the same time, one end of the polyester membrane gold-labeled antibody was connected to a fixed glass fiber, and the other end of the polyester membrane, that is, the upper end of the C-line area, was connected to filter pape...
Embodiment 2
[0027] The difference from Example 1 is that this example uses a monoclonal antibody, which is very convenient when detecting clenbuterol, clenbuterol.
[0028] The product can be used as a means of rapid on-site results for the primary screening of pigs, cattle, sheep and other livestock for the use of β-stimulants. Urine, milk, blood, muscle or visceral extracts can all be used as samples.
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