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Method for extracting yolk oil by enzyme hydrolysis-solvent extraction

A technology of enzymatic hydrolysis and egg yolk, applied in the direction of fat oil/fat production, fat production, etc., can solve the problems of industrialized large-scale production constraints, achieve the effect of high total oil yield and improve economic benefits

Inactive Publication Date: 2003-10-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] Each of the above methods has technical or cost deficiencies, and industrialized mass production is restricted to varying degrees.

Method used

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  • Method for extracting yolk oil by enzyme hydrolysis-solvent extraction
  • Method for extracting yolk oil by enzyme hydrolysis-solvent extraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Beat the eggs with fresh eggs to separate them, and stir them with egg yolks and water to prepare 10% (W / W) egg yolk liquid.

[0049] Add protease A of 1% (W / W) of substrate mass, perform enzymatic hydrolysis reaction at 37°C and pH7 for 2.5 hours, and then add protease B of 1.5% (W / W) substrate mass to continue enzymatic hydrolysis reaction for 2 hours.

[0050] Centrifuge at 90° C. and 3000 rpm for 15 minutes, the upper layer is the first part of neutral fat egg oil product, and the oil yield is 59%, and the lower layer protein hydrolyzate is a by-product.

[0051] Dehydrate the lipoprotein emulsion in the middle layer for 60 minutes at a vacuum degree of 0.1 MPa and a heating temperature of 50°C.

[0052] Add food-grade absolute ethanol to the dehydration mixture at a ratio of 1 g:gml, and extract at 50° C. for 50 min.

[0053] The extraction mixture is separated by filtration using analytical filter paper as a medium, and the raffinate remains on the filter paper a...

Embodiment 2

[0056] Add water and stir with egg yolk powder to prepare 10% (W / W) egg yolk liquid. The rest of the operating conditions are the same as

[0057] Example 1.

Embodiment 3

[0059] Add water and stir with egg yolk powder to prepare 10% (W / W) egg yolk liquid.

[0060] Add protease A with 2% (W / W) of the substrate mass. The hydrolysis reaction time is 3.5 hours. All the other operating conditions are the same as in Example 1.

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Abstract

A process for extracting yolk oil from fresh yolk or yolk powder includes enzymolyzing to obtain the neutral yolk oil and the emulsified lipoprotein liquid, and extracting from said emulsified lipoprotein liquid in alcohol to obtain the yolk oil containing phosphatide. Its advantages are high oil yield and high utilization rate of protein as by-product.

Description

technical field [0001] The invention relates to a method for extracting egg yolk oil by using an enzyme water horn-solvent extraction method, and the invention belongs to the technical field of egg oil product preparation. Background technique [0002] Since the reform and opening up, my country's poultry industry has developed rapidly, and the annual production of eggs has reached more than 20 million tons so far, making it the world's largest producer of poultry eggs for 15 consecutive years. There is a big gap compared with the proportion of deep processing of poultry eggs in Europe, America, Japan and other countries; and eggs are a kind of complete food, especially the egg yolk contains a large amount of physiologically active substances, and the development of its physiologically functional components has always attracted attention . [0003] Egg yolk is a very complex system, accounting for about 26-33% of the egg's weight. The chemical composition of egg yolk is ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10
Inventor 许学勤陈洁王辉项建琳
Owner JIANGNAN UNIV
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