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Production process of whole silkworm chrysalis powder containing lactoferrbumin and its peptide

A technology of lactoferrin and a production method, which is applied in the directions of using a carrier to introduce foreign genetic material, digestive system, medical raw materials derived from arthropods, etc.

Inactive Publication Date: 2005-01-05
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whole silkworm powder is rich in nutritional value and has some special effects. It is an excellent protein source, but there is no relevant report on whole silkworm chrysalis powder containing lactoferrin or lactoferrin peptides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] The following takes mouse lactoferrin as an example to introduce the specific operation method:

[0011] Let the mother mice lactating for 15 days be killed by necking, extract 50-100 mg of mammary gland tissue, put it in a mortar that has been baked at 200°C and cooled to room temperature, add 1 / 3 of the liquid nitrogen in the mortar, and grind it into powder And transfer it into a 1.5ml microcentrifuge tube, add 1ml Trizol solution, mix well, let stand on ice for 5 minutes, then add 0.2ml chloroform, cover the tube tightly, shake vigorously and mix well, then centrifuge at 4°C 12000rpm for 30 seconds , take the upper aqueous phase into a new microcentrifuge tube, add 0.5ml of isopropanol, mix well and let stand at -20°C for 30 minutes, centrifuge at 12000rpm for 10 minutes, discard the supernatant, wash the precipitate with 1ml of 75% ethanol and dry at room temperature , after drying, the precipitate was dissolved in 20-100 μL enzyme-free water to obtain a total RNA ...

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PUM

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Abstract

The invention is a method of producing all-pupa powder containing lactoferrin and its peptide, adopting bacilliform viruses as carrier, and using silkworm pupas as host to produce the gene engineering product with the bioactivity similar to that of the natural lactoferrin and the corresponding peptide. The producing and using course is safe and has no latent danger to human beings and animals and the environment. The all-pupa powder acting as feed additive can supplement feed proteins and simultaneously play a role in sterilizing and resisting viruses, and enhancing self-immunity of animals, and can effectively prevent and cure the diarrhea of weaner piglets, thus producing a high-quality animal product without residual antibiotics.

Description

technical field [0001] The invention relates to a preparation method of whole silkworm chrysalis powder containing lactoferrin and its peptide. Background technique [0002] Lactoferrin (Lactoferrin referred to as LF), isolated from milk in 1960, is an iron-binding glycoprotein with a molecular weight of about 80KDa. LF has the effect of transporting iron ions and sterilizing and antiviral. Its expression level in mammary glands The highest, and the highest content in human milk, the content in cow's milk is relatively small. Its unique mechanism of action has attracted people's attention, and it is separated and purified from milk and added to infant milk powder. Lactoferrin peptide (Lactoferricin, referred to as Lfcin) is the product of lactoferrin degraded by protease in the digestive tract of animals, and some of its biological activities are stronger than lactoferrin. Whole silkworm powder is rich in nutritional value and has some special eff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/20A23K50/30A23K50/60A61K35/64A61P1/12C12N15/86
Inventor 汪以真许梓荣黄海青
Owner ZHEJIANG UNIV
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