Sample pretreatment solution for immunological test and method for using the same

A technology of pretreatment liquid and immunochromatography, which is applied in the field of sample pretreatment liquid, can solve the problems such as no disclosure

Inactive Publication Date: 2005-02-09
SYSMEX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, nothing has been published about flu testing

Method used

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  • Sample pretreatment solution for immunological test and method for using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Effect of nonionic surfactant (NP40)

[0042] The implementation of this example is to determine the effect of various concentrations of non-ionic surfactant (NP40) in the sample pretreatment solution on the background on the membrane carrier for chromatography.

[0043] 1) Composition of sample pretreatment solution

[0044] In a solution (pH6.0) containing 100mM citric acid-0.4MNaCl, 10mM dithiothreitol, add Nonidet P-40 (NP40) (trade name of polyoxyethylene (9) octylphenyl ether) to make Concentrations reached 0, 0.05, 0.1, 0.2, 0.4, 0.8 (v / v)% respectively to make sample pretreatment solution.

[0045] 2) Sample and its processing

[0046] Nasal snot was collected as a sample with a cotton swab.

[0047] Immerse the mucus-containing part of the swab into a container containing 0.8 mL of the above-mentioned sample pretreatment solution, and mix it with the sample pretreatment solution to prepare a sample. Then, the sample solution after standing still was filtere...

Embodiment 2

[0054] Effects of Various Nonionic Surfactants (Background)

[0055] The implementation of this example is to confirm the effects of various non-ionic surfactants in the sample pretreatment solution on the background on the membrane carrier for chromatography.

[0056] In the solution (pH6.0) that contains 100mM citric acid-0.4MNaCl, 10mM dithiothreitol, add various nonionic surfactants as shown in Table 2 to make its concentration reach 0.1 (v / v)%, prepare into a sample pretreatment solution.

[0057] The operation process of the sample and its processing and immunochromatography is the same as that in Example 1.

[0058] The results showed that when various nonionic surfactants as shown in Table 2 were used, the color development of the background was weakened, so that the result could be judged correctly.

[0059] Table 2

[0060] Product name

[0061] Good: The membrane carrier for chromatography was not colored by the judgment time, and a blue colored zone w...

Embodiment 3

[0063] Effect of various nonionic surfactants (influenza detection)

[0064] This example is the same as Example 2, to confirm the effects of various non-ionic surfactants in the sample pretreatment solution on immunochromatography.

[0065] The operation process of sample pretreatment solution, sample and its processing, and immunochromatography is the same as that in Example 2.

[0066] The results showed that after using various nonionic surfactants as shown in Table 3, the results under all surfactants were judged as positive.

[0067] table 3

[0068] Product name

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Abstract

Provided is a pretreatment liquid in a specimen and a pretreatment method required for accurate and simple inspection for inspecting influenza virus by immune chromatography. A specimen, sampled from an organism for inspecting influenza, such as pituita, phlegm, and a pharynx wiping liquid, is treated by a pretreatment liquid containing a nonionic surface active agent and 0.3M alkali metal ions.

Description

technical field [0001] The invention relates to a sample pretreatment liquid for immunochromatographic detection, and more specifically, to a sample pretreatment liquid for influenza virus detection. Background technique [0002] Influenza virus is an RNA virus with a diameter of 80 to 120 nm, has an outer shell, and the surface is covered with protrusions of two enzyme proteins, HA (hemagglutinin) and NA (neuraminidase). HA is a hemagglutinating antigen, which plays an important role in the process of binding to sialic acid on the cell surface when it is adsorbed on human cells and invading the human body, and the virus particles enter the interior of the cells. NA is used to cut off sialic acid when the virus particles leave the cell surface in the late stage of infection, and play a role in acquiring infectivity. Antigenicity is determined by HA and NA together, and can be roughly divided into three types: A, B, and C. Type A is also known to have subtypes such as the H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/558G01N33/569
CPCG01N33/558G01N33/56983G01N33/54393G01N2333/11
Inventor 一口毅古谷基安岐昌子
Owner SYSMEX CORP
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