Three spiral lentinan with anti-cancer activity and its preparation and use

An anti-tumor activity, lentinan technology, applied in the fields of polymer physics and biology, can solve the problems of curative effect restriction and the like, and achieve the effects of low cost, high anti-tumor activity, and improved immune function

Inactive Publication Date: 2005-05-11
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fruiting bodies of mushrooms are directly used as traditional Chinese medicine, and the curative effect is restricted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Purchasing commercially available mushroom fruit bodies produced in Suizhou, Hubei, the mushroom fruit body fragments were Soxhlet extracted with ethyl acetate and acetone for 8 hours each. Then it was extracted three times with 0.9% NaCl aqueous solution at room temperature: soaked in 0.9% NaCl aqueous solution overnight, centrifuged, and the residue was collected. The residue was extracted three times with hot water (100°C) for half an hour each time, centrifuged, and the residue was collected; the residue was sequentially used 0.4% NaOH / 0.02% NaBH 4 , 0.8%NaOH / 0.02%NaBH 4 And 5%NaOH / 0.05%NaBH 4 The aqueous solution was extracted once at room temperature, centrifuged, and the supernatant was collected. It was neutralized to pH=7 with 1M HOAc aqueous solution at room temperature, centrifuged, and the supernatant was collected. After the supernatant is concentrated, use 30% H 2 O 2 Decolor to light yellow, remove free protein by Sevag method, repeat 9 times or more. Then, i...

Embodiment 2

[0021] The mushroom fruit bodies used were cultivated by 4 hybrid strains coded No.2, No.36, No.39 and No.50 developed by the Fungus Research Laboratory of Huazhong Agricultural University and sold to the public (available to the public) . The 4 kinds of shiitake mushroom fruit body fragments were subjected to Soxhlet extraction with ethyl acetate and acetone for 8 hours respectively. Then it was extracted three times with 0.9% NaCl aqueous solution at room temperature: soaked in 0.9% NaCl aqueous solution overnight, centrifuged, and the residue was collected. The residue is extracted three times with hot water (120°C) in a pressure cooker for half an hour each time, centrifuged to collect the residue; the residue is 5% NaOH / 0.05% NaBH 4 The aqueous solution was extracted once at room temperature, centrifuged, and the supernatant was collected. It was neutralized to pH 6-8 with 36% HOAc aqueous solution at room temperature, centrifuged, and the supernatant was collected. After the...

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PUM

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Abstract

This invention relates to three-spiral lentina with beta-(1-3)-D-glucan having 20-30%(1-6) chain connected glucose side chains as main chain. It shows three chains in water, with average molecular weight of 20,000-200,000. It is produced by: cutting dry shiitake, removing fat by Suo extraction, soaking in normal saline, centrifuging, extracting remains at 100-130 degrees C, centrifuging, extracting remains in water solution of NaOH/NaBH4, adding acetic acid into extracting liquid to neutralize, decoloring supernatant, removing free protein, dialyzing, concentrating, and frozen drying. It has higher anti-tumor activity and can be used to prepare medicines or health-care products for improving immune ability and antitumor.

Description

Technical field [0001] The invention relates to a triple helix lentinan with anti-tumor activity, and a preparation method and application thereof. It belongs to the field of polymer physics and also belongs to the field of biology. Background technique [0002] In the early 1960s, Chihara discovered that the polysaccharides extracted from the fruit bodies of lentinus edodes had anti-tumor activity (Nature, 1969, 222, 687; 1970, 225, 943; Cancer Res., 1970, 30, 2776.). Since then, polysaccharide research has been affected. Great attention. Studies since the 1980s have shown that fungal polysaccharides are an immunomodulator, which can enhance the phagocytic function of macrophages and leukocytes, induce the production of tumor necrosis factor (TNF) and leukocytes, thereby improving the body’s immune function. Diseases that endanger human health, such as immune disorders, cancer, diabetes, hypertension, hepatitis, pneumonia, etc., have certain curative effects, and have no toxic s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/716A61P35/00C08B37/00
Inventor 张俐娜许小娟俄尼尔李雪莲
Owner WUHAN UNIV
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