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Swing breeding and respiratory syndrome suicide DNA vaccine and use

A technology for respiratory syndrome and DNA vaccines, applied in the field of animal virology and zoonosis, can solve the problem of low immune dose

Inactive Publication Date: 2005-07-20
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on this new type of vaccine is still in the early stage of research, but it has been reported that the "suicide" DNA vaccines of influenza virus and human herpes simplex virus constructed by this method can induce higher levels than conventional DNA vaccines after immunizing experimental animals with mice. Humoral immunity and cellular immunity, and the immune dose is 100-1000 times lower than that of conventional DNA vaccines, demonstrating the huge development potential and good application prospects of "suicide" DNA vaccines

Method used

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  • Swing breeding and respiratory syndrome suicide DNA vaccine and use
  • Swing breeding and respiratory syndrome suicide DNA vaccine and use
  • Swing breeding and respiratory syndrome suicide DNA vaccine and use

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Embodiment Construction

[0070] Below in conjunction with accompanying drawing, the present invention will be further described:

[0071] 1. Construction of conventional DNA vaccine expression plasmid pCI-ORF5

[0072] Using pMD-ORF5 as a template, P52S and P52R as primers to amplify the ORF5 gene, the purified amplified product was digested with XhoI and XbaI, and cloned directly into the corresponding site of the eukaryotic expression vector pCI-neo to obtain the recombinant plasmid pCI- ORF5. For its construction process, see figure 1 .

[0073] 2. Construction of "suicide" DNA vaccine expression vector pSCA

[0074] Using pCI-neo as a template, P1 and P2 as primers to amplify the SV40 poly(A) sequence, digest with SpeI and XbaI after purification, recover a fragment with a size of about 250bp, insert it into the SpeI site of vector pSFV1, and obtain a recombinant plasmid pSFVA. Then use pCI-neo as a template and P3 and P4 as primers to amplify the HCMV IE Enhancer / Promoter fragment. After the ...

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Abstract

The present invention discloses a 'suicidal' DNA vaccine and its application for preventing and curing swine reproduction and respiration syndrome. It is characterized by that it is constructed by inserting the main immunogenic gene ORF5 of swine reproduction and respiration syndrome virus into BamHI site of 'suicidal' DNA vaccine expression vector pSCA, at the same time the expression vector pSCA suitable for preparing said 'suicidal' DNA vaccine also is constructed. Besides said invention also provides a method for preparing said expression vector pSCA.

Description

technical field [0001] The invention relates to the technical field of animal virology and animal infectious disease. Specifically, it relates to a "suicide" DNA vaccine for porcine reproductive and respiratory syndrome, and also relates to the application of the vaccine in preventing and controlling porcine reproductive and respiratory syndrome. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS for short, hereinafter referred to as PRRS) is a new viral infectious disease discovered in recent years. Disorders and respiratory disease and high mortality in pigs of all ages are characterized. The disease was first reported in the southern United States in 1987, and soon spread to the Midwest and spread rapidly across the United States. Subsequently, some countries such as Canada, Germany, and the Netherlands also successively broke out the disease (Bilodeau R, etc., Porcine reproductive and respiratory syndro...

Claims

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Application Information

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IPC IPC(8): A61K39/12A61K48/00A61P31/12
Inventor 陈焕春方六荣肖少波金梅林吴斌刘正飞江云波牛传双
Owner HUAZHONG AGRI UNIV
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