New tracer based on nucleic acid and application thereof

A tracer and nucleic acid technology, applied in the direction of sampling devices, etc., can solve the problems of multi-time, inability to provide, and inability to realize multiple snapshot reproductions, etc.

Inactive Publication Date: 2005-12-14
GUANTASO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0026] All of these methods are either qualitative or require too much time to collect samples to give quantitative data or provide an accurate snapshot of a given location in

Method used

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  • New tracer based on nucleic acid and application thereof
  • New tracer based on nucleic acid and application thereof
  • New tracer based on nucleic acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0124] 1. Examples of oligonucleotides and native DNA and their corresponding primers

[0125] (1) Sequence of 60 nucleotides and their primers

[0126] CATGAATTCC GCTGCATACC AATGTGTAAG 30

[0127] CTAGATCAGT CAGGACTGAT CGAATTCCAG 60

[0128] Forward primer: CATGAATTCC GCTGCATACC 20

[0129] Reverse primer: CTGGAATTCG ATCAGTCCTG 20

[0130] (2) Sequence of 100 nucleotides and their primers

[0131] GGCTACCGCT CGCATACTAA TGGTGTACGA 30

[0132] CAGCTCGAGC TATCTGCGTA CGTATCGTAT 60

[0133] GCATGCCGCG CAGTAGGAGC ACTATATCGA 90

[0134] GCTATCCATC 100

[0135] Forward primer: CGCTCGCATA CTAATGGTGT A 21

[0136] Reverse primer: TATAGTGCTC CTACTGCGCG G 21

[0137] (3) Sequence of 100 nucleotides and their primers

[0138] GGCTAGGGGA TAGCTACTAG CCGTTTATTG 30

[0139] CGCTATCTAC GTACTGATCC GTAGACGCGT 60

[0140] ACATCAGCTAGCGTGCTAGCTACATCGACG90

[0141] CGCATTACGA 100

[0142] Forward primer: TACTAGCCGT TTATTGCGCT 20

[0143] Reverse primer: TAATGCGCGT GCATGTAGCT 20

[0...

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Abstract

The present invention relates to a method of testing whether a facility is effective in preventing the spread of an infectious disease by a) releasing in or near the facility an effective amount of a biological tracer to simulate the spread of a pathogen that causes the infectious disease; and b) Detecting the presence or amount of biological tracers to determine the spread of biological tracers in the environment of the facility. The present invention also provides a method and apparatus for generating aerosols that simulate human sneezing and coughing, so as to more realistically determine the spread of infectious diseases from such sources.

Description

[0001] This application claims priority to US Provisional Patent Applications Serial No. 60 / 493,182, filed August 7, 2003, and US Provisional Patent Applications Serial No. 60 / 510,207, filed October 10, 2003. field of invention [0002] In order to prevent cross-infection in hospitals caused by aerosols generated by patients, medical interventions or other activities, each ward and even each bed should be individually ventilated. The present invention provides methods for determining whether the above conditions are met. In addition, the present invention provides a new tool for experimental simulation, determination of the spread of respiratory infectious disease outbreaks and tracking of contamination sources of surface, groundwater and air. [0003] The present invention further provides portable and lightweight apparatus and methods for generating aerosols loaded with viral and bacterial simulants to simulate the ejection of lung gases produced by humans during activities ...

Claims

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Application Information

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IPC IPC(8): G01N1/22
Inventor 唐舜荣梁纯
Owner GUANTASO
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