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Gas phase chromatography analysis method for micro ethanol in human blood

A gas chromatographic analysis, human body technology, applied in the analysis of materials, measuring devices, material separation, etc., can solve the problems of ethanol loss, reduce the sensitivity of the method, and the quantitative accuracy depends on the automation of headspace equipment, so as to reduce the pretreatment work Effect

Inactive Publication Date: 2006-01-04
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the headspace method is the most reported. This method does not introduce interfering substances, but the quantitative accuracy depends more on the automation of the headspace equipment, and simple equipment often has poor quantitative accuracy.
The solvent extraction method has simple equipment, but the introduction of a solvent is likely to cause interference and difficulties in chromatographic separation, and it is also easy to cause loss of ethanol during concentration
The protein precipitation method is to add reagents to the blood supernatant after centrifugation to precipitate the protein in it. The purpose is to protect the chromatographic column. The method is relatively simple, but the introduced reagents may also cause interference or pollute the chromatographic system.
The dilution method reduces the contamination of the sample injection system and chromatographic column by the blood sample due to the dilution of the blood, but at the same time reduces the sensitivity of the method

Method used

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  • Gas phase chromatography analysis method for micro ethanol in human blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Preparation of chromatographic column

[0039] Clean the empty tube of the glass or stainless steel chromatographic column and blow it dry with nitrogen, fill a little glass or quartz fiber at the end of the column and connect it to a vacuum pump, connect the glass funnel at the head end of the column, and pour the chromatographic grade divinylbenzene porous polymer stationary phase into the glass funnel Inside, while pumping air at the end of the column, tap the column tube with appropriate strength, put the stationary phase into the empty tube of the chromatographic column in batches, and fill the end of the column head with a little glass or quartz fiber to seal it to prepare a special chromatographic column. The chromatographic column is 1 meter long and has an outer diameter of φ3 mm.

[0040] 2) Aging of the chromatographic column:

[0041] Install the packed chromatographic column into the gas chromatographic oven. One end of the column head is connected to t...

Embodiment 2

[0048] 1) Chromatographic column preparation

[0049] Clean the empty tube of the glass or stainless steel chromatographic column and blow it dry with nitrogen. Fill a little glass or quartz fiber at the end of the column and connect it to a vacuum pump. Connect a small glass funnel to the end of the column head. Pour the stationary phase of chromatographic grade divinylbenzene porous polymer into the glass. In the funnel, tap the column tube with appropriate strength while pumping air at the end of the column, and put the stationary phase into the empty tube of the column in batches, leaving 10 cm in the column head to fill and load 5% methyl polysiloxane in the same way The white diatomite stationary phase is filled with a little glass or quartz fiber to separate the two stationary phases. After the column is installed, a little glass or quartz fiber is filled at one end of the column head to seal and prepare a chromatographic column with a pre-column. The chromatographic co...

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Abstract

The gas chromatographic analysis method for micro ethanol in human blood has chromatographic level porous divinylbenzene polymer as fixed phase or two kinds of fixed phases of white diatomite supporting 5 % polymethyl siloxane and chromatographic level porous divinylbenzene polymer to prepare chromatographic column; and the chromatographic separation conditions including column temperature of 150 deg c, vaporizing chamber temperature of 150 deg c,, FID detector temperature of 160 deg c, carrier gas flow rate of 22 ml / min, post-column pressure of 0.14 MPa, hydrogen flow rate of 42 ml / min, air flow rate of 270 ml / min, and top sampling of 0.5-5 microliter. The present invention has simple operation, high accuracy, no need of special treatment of blood sample and analysis period of only several minutes.

Description

technical field [0001] The invention relates to a gas chromatography analysis method for trace ethanol in human blood. Background technique [0002] Drunk driving is one of the important hidden dangers of urban traffic safety, so the accurate quantitative detection of ethanol (alcohol) in human blood is very important. Ethanol enters the blood through the digestive system of the human body and spreads all over the body. The main effect on the human body is to paralyze and inhibit the central nervous system, and the strength of the effect is closely related to the ethanol content in the blood. Due to the complex process of diet and metabolism, trace amounts of ethanol can sometimes be detected in the blood of normal people who do not drink alcohol. Therefore, many countries in Europe and the United States have legal standards for the minimum concentration of ethanol in the driver's blood, generally 50-80mg / 100ml. my country's current laws and regulations strictly prohibit d...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/60
Inventor 梁冰李辰
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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