Apparatus and method for quick detection of surface cleanness degree and microbe contamination

A technology for microbial contamination and surface cleaning, applied in biochemical equipment and methods, microbial determination/inspection, measuring devices, etc., can solve problems such as high pollution value, low fluorescence value, and ineffective use

Inactive Publication Date: 2006-07-19
卢麟麟
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  • Summary
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Problems solved by technology

[0009] However, because microorganisms are in a natural state and environment, they often do not get enough nutrients or cannot effectively use food or some nutrients on the surface under certain conditions, which will inevitably lead to bacteria consuming their own ATP to maintain Self-survival (ATP hydrolysis reaction or other enzymatic reactions will consume a lot of ATP), unti

Method used

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  • Apparatus and method for quick detection of surface cleanness degree and microbe contamination
  • Apparatus and method for quick detection of surface cleanness degree and microbe contamination
  • Apparatus and method for quick detection of surface cleanness degree and microbe contamination

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Embodiment Construction

[0055] When implementing the present invention, the ATP-free cotton swab is wetted with ATP-free 0.05% Tween20 or a mixed solution containing 10mM PBS and 0.05% Tween20, which helps to completely remove microorganisms and food residues on the surface of the detected object Wipe it down. Then smear or wipe the cotton swab on an area of ​​appropriate size on the surface to be tested, then insert the cotton swab into the pen-type detection chamber, and press down on the handle of the cotton swab to make the cotton swab pierce the aluminum foil composite film below, making it compatible with the Surface cleanliness and microbial contamination rapid test reagent (QT) contact, and shake to make it fully mixed. At this time, the cleavage and extraction system in the QT reagent will lyse the microbial cells and release the ATP, while the ATPase inhibitor will inhibit the activity of the ATPase released from the microorganism. ) react with pyrophosphate (PPi) to reduce the hydrolyzed ...

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Abstract

The invention relates to a method and apparatus for fast testing the surface cleaning degree and microorganism pollution. The ATP sulfurylase can promote the PPi on the condition of existing adenosine-5'-phosphosulfate APS to generate ATP so as to reduction the hydrolysis pyrophosphoric acid ATP. It adds firefly luciferase stabilizer to simplify the preserving condition and adds adenosine triphosphatase restrainer to improve the test accuracy and sensibility.

Description

Technical field: [0001] The present invention relates to a reagent set and a device, in particular to a reagent set for rapid detection of surface cleanliness and microbial contamination and a device for detection using the reagent set. Meanwhile, the present invention can also be applied to the sterilized operation interface, The assembly line and liquid pollution detection of food production enterprises belong to the field of biochemistry and bioluminescence. Background technique: [0002] Food Hygiene Certification System - Hazard Critical Control Point (HACCP) is being introduced into the food industry worldwide. Many countries, such as the member states of the European Union, have listed it as a statutory measure. The aim is to include the key factors that may affect food safety into the scope of monitoring, and to use rapid methods for sanitation testing to allow time for improvement measures. The Ministry of Health of my country has also issued a guideline on "Imple...

Claims

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Application Information

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IPC IPC(8): G01N21/94G01N21/76C12Q1/00
CPCG01N21/763
Inventor 卢麟麟
Owner 卢麟麟
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