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Method for producing compound bioactive peptide utilizing casein enzyme process

A bioactive peptide and casein technology, applied in the direction of fermentation, etc., can solve the problems of complex process and high production cost, and achieve the effect of simple process, mild conditions and strong hydrolysis ability

Inactive Publication Date: 2006-08-09
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN1208565 discloses a kind of " preparation method of milk protein active peptide ", and CN1101471C discloses a kind of " preparation method of soybean protein active peptide ", and these methods are raw material with fresh milk or skim milk powder or soybean, and production cost is higher, and technology is also comparatively low. complex

Method used

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  • Method for producing compound bioactive peptide utilizing casein enzyme process
  • Method for producing compound bioactive peptide utilizing casein enzyme process
  • Method for producing compound bioactive peptide utilizing casein enzyme process

Examples

Experimental program
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Embodiment Construction

[0018] (1) Preparation of enzymatic hydrolysis substrate:

[0019] Add 320ml of distilled water to 80g of casein to swell, adjust the pH to 8.0-9.0 with 6mol / L NaOH, heat to 60°C to dissolve the casein, then adjust the pH to 7.2 with 1mol / L HCl, and finally adjust the volume to 1000ml.

[0020] (2) Enzymolysis:

[0021] 0.8 g of trypsin and 1.2 g of dispase were uniformly dissolved in the prepared substrate solution (slowly stirred until uniform while adding). The enzymatic hydrolyzate was reacted in a constant temperature water bath at 55°C for 6 hours (the hydrolyzate was often stirred or stirred automatically 50 times / min during the reaction).

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Abstract

This invention puts forward a method for producing compound biological active peptides by a casein enzyme method including preparing an enzymolysis substrate with casein, utilizing the compound trypsase and a neutral proteinase to carry out enzymolysis and controlling the hydrolyzed degree to 18-20%, then eliminating the enzymes, vacuum depressing and concentrating, the concentrated fluid is sprayed and dried to get the white-ivory-white powder compound active peptide product.

Description

technical field [0001] The invention relates to a method for producing compound bioactive peptides by using caseinase method. Background technique [0002] Hydrolysis of proteins by proteases by enzymatic hydrolysis is a common method to obtain bioactive peptide products. CN1208565 discloses a kind of " preparation method of milk protein active peptide ", and CN1101471C discloses a kind of " preparation method of soybean protein active peptide ", and these methods are raw material with fresh milk or skim milk powder or soybean, and production cost is higher, and technology is also comparatively low. complex. Contents of the invention [0003] The purpose of the present invention is to propose a new method for producing compound biologically active peptide products with strong biological activity and high safety by utilizing the raw material of casein with wide sources and low price by compound enzymatic hydrolysis. [0004] Processing step of the present invention is as ...

Claims

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Application Information

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IPC IPC(8): C12P21/00
Inventor 许国焕吴月嫦舒美艳
Owner GUANGDONG INST OF MICROORGANISM
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