Al type grippal virus fluorescent augmentation detection kit and detection method
A detection kit, influenza virus technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of high price, easy contamination in detection, low sensitivity, etc., and achieve simple and convenient sampling and convenient and accurate judgment. , the effect of high sensitivity
Inactive Publication Date: 2010-12-15
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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Problems solved by technology
In recent years, rapid diagnosis kits for influenza virus have been developed abroad. The principles include fluorescence method, enzyme labeling method, gold standard method, etc. Although they can achieve the purpose of rapid diagnosis, their sensitivity is far lower than that of virus isolation method, and false negative Higher, coupled with high price, it is rarely used in our country
The RT-PCR detection of influenza virus is more sensitive than the classic virus isolation method, and the result can be obtained within 6-7 hours, but it has the disadvantages of easy contamination and high false positive rate
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Embodiment 1
[0064] Suspected influenza outbreaks in Zhejiang Province and 8 patient gargles submitted to influenza monitoring hospitals were tested using the TaqMan fluorescent quantitative RT-PCR method of the present invention, conventional RT-PCR methods and virus isolation methods for detection, and 5 of them were positive for virus isolation, RT -PCR was positive in 6 cases, and TaqManRT-PCR was positive in 7 cases. The positive results of these three tests were consistent. For cases with positive fluorescent RT-PCR but negative virus isolation, in order to rule out the possibility of false-positive results of fluorescent RT-PCR, we tested influenza virus hemagglutination-inhibiting antibody (HI) on double sera from patients in the early stage of onset and recovery period, and the results were A The HI antibodies of type 1 influenza virus all increased by ≥4 times, and the serology was completely consistent with the results of TaqMan RT-PCR.
[0065] The composition of the TaqMan flu...
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The present invention provides a kind of influenza A1 virus fluorescence amplification detection kit and method for detecting influenza A1 virus by using said kit. Said kit includes influenza A1 virus hemagglutinin gene standard product, fluorescence amplification detection reagent and DNA polymerase and reverse tracriptase. The fluorescence amplification detection reagent mainly includes one-step method RT-PCR fuffer fluid, specific amplification primer and specific probe and deoxynucleoside triphosphate mixture. Said method can directly detect pathogen nucleic acid, and can detect specific gene containing influenza A1 virus.
Description
(1) Technical field [0001] The invention relates to a fluorescent amplification detection kit and a detection method of type A1 influenza virus. (2) Background technology [0002] The clinical symptoms of influenza (flu) are easily confused with the common cold, and finally must be confirmed by laboratory methods. In recent years, rapid diagnosis kits for influenza virus have been developed abroad. The principles include fluorescence method, enzyme labeling method, gold standard method, etc. Although they can achieve the purpose of rapid diagnosis, their sensitivity is far lower than that of virus isolation method, and false negative Higher and more expensive, it is rarely used in our country. RT-PCR detection of influenza virus is more sensitive than the classic virus isolation method, and the result can be obtained within 6-7 hours, but it has the disadvantages of easy contamination and high false positive rate. [0003] Fluorescent quantitative PCR technology developed ...
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IPC IPC(8): C12Q1/68
Inventor 严菊英卢亦愚茅海燕冯燕史雯周敏李敏红
Owner ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION