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Parents composition method for distant hybrid of peony and Chinese herbaceous peony

A technology of distant hybridization and combination method, which is applied in the field of parental combination of distant hybridization of peony and peony, which can solve the problems of unused wild germplasm, limited use of germplasm, and reduced potential of gene resource mining.

Inactive Publication Date: 2007-04-18
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these artificial crosses are limited to the same subgroup or within the same breed group. The germplasm used in the hybridization is limited, and the wild germplasm is almost never used, and the long-term close hybridization makes the potential of genetic resources in the breed group tapped. Gradually decreased, so peony distant hybrid breeding is imperative

Method used

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  • Parents composition method for distant hybrid of peony and Chinese herbaceous peony
  • Parents composition method for distant hybrid of peony and Chinese herbaceous peony

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) emasculate and bag the flowers to be hybridized 1 to 2 days before seedling 1# blooms.

[0047] (2) Pick the flower buds of 'Jindi' that are about to open and bring them back indoors, remove the petals, dry them for 24 hours, pat them until the pollen is completely scattered, collect them, put them into a clean weighing bottle, and put them in the refrigerator at -7 or -18°C Store for later use. The pollen germination rate was detected by hanging drop culture method before pollination.

[0048] (3) When the stigma of the flower that seedling 1# will hybridize secretes mucus in a large amount, choose the pollen of 'Jindi' for 3 consecutive days in the windless morning for 3 times. After 15 days go to the bag.

[0049] (4) Seeds gradually mature from the end of July to mid-August. When the carpel roe is slightly cracked, the seeds are removed and the seed setting rate is counted.

[0050] (5) Seeds were soaked in 2% NaClO solution for 15-20 minutes, washed with disti...

Embodiment 2

[0052] (1) emasculate and bag the flowers to be hybridized 1 to 2 days before seedling 1# blooms.

[0053] (2) Pick the flower buds of 'Scholar Holding Ink' that are about to open and bring them back indoors, remove the petals, dry them for 24 hours, pat until the pollen is completely scattered, collect them, put them into a clean weighing bottle, and put them in the refrigerator for -7 or -18 Store at ℃ for later use. The pollen germination rate was detected by hanging drop culture method before pollination.

[0054] (3) When the stigma of the flower that seedling 1# will hybridize secretes a large amount of mucus, choose the pollen of 'Scholar Holding Ink' for 3 consecutive days in the windless morning for 3 times. After 15 days go to the bag.

[0055] (4) Seeds gradually mature from the end of July to mid-August. When the carpel roe is slightly cracked, the seeds are removed and the seed setting rate is counted.

[0056] (5) Seeds were soaked in 2% NaClO solution for 15-...

Embodiment 3

[0058] (1) emasculate and bag the flowers to be hybridized 1 to 2 days before seedling 1# blooms.

[0059] (2) Pick the flower buds of 'Golden Era' that are about to open and bring them back indoors, remove the petals, dry them for 24 hours, pat them until the pollen is completely scattered, collect them, put them into a clean weighing bottle, and put them in the refrigerator at -7 or -18°C Store for later use.

[0060] (3) When the stigma of the flower that seedling 1# will hybridize secretes a large amount of mucus, choose the pollen of 'Golden Era' for 3 consecutive days in the windless morning for 3 times. After 15 days go to the bag.

[0061] (4) Seeds gradually mature from the end of July to mid-August. When the carpel crab roe is slightly cracked, the seeds are peeled off, and the seed setting rate is counted.

[0062] (5) Seeds are soaked in 0.01% GA3 solution for 5 hours, dried and sown in the field. Routine management after emergence in the following year.

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Abstract

A parents combining method for the distant hybridization between tree peony and peony includes such steps as choosing parents from Paeonialactiflora seedling cluster No.1, and the distant-hybridized peony varieties: 'L' Espe rance, Souvenir de Maxime cornu, High Noon, Golden Era, P.rockii and P.delavayi, bybrid combination, collecting pollen, germination testing, hybridizing, chemical treating, germination of hybridized seed, and seedling management.

Description

technical field [0001] The invention relates to a parent combination method for distant hybridization of peony and peony. Background technique [0002] Paeoniaceae is divided into three groups: peony group, peony group and North American peony group, and the peony group is divided into two subgroups: leathery disk and fleshy disk. The 8 natural wild species in the group are all native to China. The cultivated varieties Also originated in China. Hybrid breeding has made a great contribution to the prosperity of peony and peony all over the world, especially distant hybridization, which is the most important source of variation, and many excellent varieties are derived from it. [0003] The distant hybrid breeding of peonies and peonies began in France in the 19th century and has a history of more than 100 years. A large number of new varieties have been bred and successfully rewritten the variety structure and appearance of peonies and peonies in the world, which has caused ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01C1/00
Inventor 成仿云何贵梅
Owner BEIJING FORESTRY UNIVERSITY
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