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Peptide for skin regeneration or wound treatment and use thereof

a technology for wound treatment and skin regeneration, applied in the direction of peptides/protein ingredients, drug compositions, peptides, etc., can solve the problems of low recovery yield, low therapeutic effect of egf, and high cost, and achieve excellent whitening effect, promote wound healing, and increase the production of collagen

Active Publication Date: 2019-01-29
KINE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0024]The novel peptide according to the present invention not only promotes the wound healing by increasing the production amount of collagen in dermal fibroblasts but also has an excellent whitening effect by inhibiting the production amount of melanin and tyrosinase activity of melanoma cells, and consists of peptides having a very small size to minimize side effects according to administration of external substances of very small peptides. As a result, it is expected that the novel peptide can be used as an active substance that can replace existing skin regeneration or would therapeutic agents.

Problems solved by technology

It takes a lot of time, money, and labor to obtain by direct purification.
Also, the over-expression method in the bacteria has a problem in that the recovery yield is very low due to the low expression level in the cells and proteolytic enzyme of the bacteria.
In addition, the EGF has been reported to exhibit a low therapeutic effect on chronic wound sites, and it has been difficult to commercialize the EGF due to the high price to the efficiency due to a temperature and a short half-life due to the proteolytic enzyme.
Under such a background, development of a novel therapeutic agent having an effective therapeutic effect while minimizing side effects of conventional drugs for skin regeneration or wound healing has been required and has been actively studied (Korean Patent Publication No. 10-2015-0135743), but it is still not enough.

Method used

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  • Peptide for skin regeneration or wound treatment and use thereof
  • Peptide for skin regeneration or wound treatment and use thereof
  • Peptide for skin regeneration or wound treatment and use thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

on of Peptide

[0071]In Example 1, peptides in Table 1 below were prepared. Thereafter, the synthesized peptides were purified using a high-performance liquid chromatography (SHIMADZU Prominence HPLC) and a column used a Shiseido capcell pak C18 column (4.6×50 mm) Further, the mass of the synthesized peptide was confirmed using a mass spectrometer (HP 1100 series LC / MSD).

[0072]

TABLE 1NumberAmino acid sequence1REGRT (SEQ ID NO: 1)2REGRTREGRT (SEQ ID NO: 2)

example 2

n of Production Ability of Collagen of Human Dermal Fibroblasts

[0073]Collagen is a main ingredient of skin connective tissues, and increased collagen expression is essential in wound healing such as a gash or skin regeneration. As a result, in Example 2, the production ability of collagen of human dermal fibroblasts (HDF) according to the treatment of the peptide (peptide 1) prepared in Example 1 was evaluated by an experimental method disclosed in the existing document (J. Biol. Chem. 1993 268(14): 9941-9944). First, the human dermal fibroblasts were cultured in a DMEM medium added with 10% fetal bovine albumin (FBS), and the cultured cells were divided into a 6-well plate at a concentration of 2×105 cell / well and cultured for 24 hours, and then the medium was replaced with 2% FBS. After 24 hours from this, the cells were treated with the peptide (100, 10, 1 ng / ml) of the present invention, and thereafter, an amount of procollagen secreted to the culture medium was measured by usin...

example 3

n of Production Ability of Melanin of Melanoma Cells

[0075]The color of the skin is determined by an amount of melanin pigment produced by melanoma cells, and many studies have been conducted to improve abnormal pigmentation of the skin. Particularly, excessive pigmentation caused by chronic gashes is considered as a major medical problem, but no definitive treatment method has yet been developed. Thus, in Example 2, the production ability of melanin of a melanoma cell line according to the treatment of the peptide (peptide 1) prepared in Example 1 was evaluated by an experimental method disclosed in the existing document (Life Sci. 2013 Aug. 14; 93(5-6):226-32). The melanoma cell line, B16F10 cells, was cultured in a DMEM medium added with 10% fetal bovine albumin, and the cultured cells were divided into a 12 well-plate at a concentration of 1×105 cells / well. After the culture for 24 hours, α-MSH (10 uM) promoting melanin synthesis and the peptide (1, 10, and 100 ng / ml) of the pres...

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Abstract

The present invention relates to a peptide for skin regeneration or wound healing and a use thereof. The novel peptide according to the present invention not only promotes the wound healing by increasing the production amount of collagen in dermal fibroblasts but also has an excellent whitening effect by inhibiting the production amount of melanin and tyrosinase activity of melanoma cells, and consists of peptides having a very small size to minimize side effects according to administration of external substances of very small peptides. As a result, it is expected that the novel peptide can be used as an active substance that can replace existing skin regeneration or would therapeutic agents.

Description

TECHNICAL FIELD[0001]The present invention relates to a peptide for skin regeneration or wound healing and a use thereof.[0002]The present invention is achieved by the project number of 1711030975 under the support of the governmental department in Korea, the research management organization of the project is the Korea Research Foundation, the name of the research business is the discovery business of creative materials, the name of the research project is the development of biodegradable skin regeneration material based on the functional immune technology, and the research period is from Dec. 4, 2015 to Apr. 30, 2016.[0003]This application claims priority to and the benefit of Korean Patent Application No. 10-2016-0034327 filed in the Korean Intellectual Property Office on Mar. 23, 2016, the entire contents of which are incorporated herein by reference.BACKGROUND ART[0004]The skin is a primary barrier of the human body and protects the organs in the body from stimulation caused by ...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A61K38/08A61Q19/08C07K7/08C07K7/06A61Q19/02A61K8/64A61K38/10A61K47/10
CPCA61K38/08A61K8/64A61K38/10C07K7/08A61Q19/02A61Q19/08C07K7/06A61K47/10A61K38/00A61K9/0014A61P17/00A61P17/02A61P43/00A61K8/02A61K9/00A61Q19/00
Inventor CHO, DAE HOGIL, MIN CHANLEE, SO MIKIM, MYUN SOO
Owner KINE SCI CO LTD
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