Methods for producing immunoglobulins containing protection proteins in plants and their use

a technology of protection protein and immunoglobulin, which is applied in the field of producing immunoglobulins containing protection protein in plants and their use, can solve the problems of unsatisfactory side effects, immunoglobulin must be functional in very harsh environments, and the mechanism of synthesis and assembly of these secretory immunoglobulins such as siga or sigm is extremely complex

Inactive Publication Date: 2002-10-31
THE SCRIPPS RES INST
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotic treatment targeted to a single pathogen often involves eradication of a large population of normal microbes, and this can be undesired side effects.
First, the immunoglobulin must be functional in very harsh environments, such as the gastrointestinal tract.
The mechanism of synthesis and assembly of these secretory immunoglobulins, such as SIgA or SIgM is extremely complex.
The mechanism by which these cells assemble and secrete the secretory immunoglobulin is extremely complex and requires a unique microenvironment provided, for example, by mucosal tissues.
The targeting of the production of immunoglobulins in transgenic organisms, such as mice, is extremely difficult and transgenic organisms made from fungus or plants do not contain the proper cell types and mucosal microenvironment to produce secretory immunoglobulins.
This elaborate process required for natural secretory immunoglobulin assembly is extremely difficult to duplicate in cell culture or transgenic organisms.
Thus, one particular method of introducing genes into a particular eukaryotic cell or plant species may not necessarily be the most effective for another eukaryotic cell or plant species.
Agrobacterium-mediated transformation of leaf disks and other tissues appears to be limited to plant species that Agrobacterium tumefaciens naturally infects.
Commonly used homogenization techniques which are not appropriate for antibody extraction involve the use of high speed blades or cylinders which explosively destroy all plant structures.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods for producing immunoglobulins containing protection proteins in plants and their use

Examples

Experimental program
Comparison scheme
Effect test

example 7

Production of Immunoglobulins Containing Protection Proteins

[0310] Four transgenic Nicotiana tabacum plants were generated to express (1) a murine monoclonal immunoglobulin kappa chain having the antigen binding site of the Guy's 13 light chain, (2) a hybrid IgA / G murine immunoglobulin heavy chain containing C.gamma. and C.alpha. chain domains and the antigen binding site of the Guy's 13 heavy chain, (3) a murine J chain and (4) protection protein comprised of amino acids 1-606 of rabbit polyimmunoglobulin receptor and did not contain amino acids 627-675 of the rabbit polyimmunoglobulin receptor. See, Example 1. Successive sexual crosses between these plants resulted in simultaneous expression of all four protein chains in the progeny plants. In some cases, back crossing was used to produce homozygous plants. The four recombinant polypeptides were assembled into a functional, high molecular weight immunoglobulin containing a protection protein of approximately 470,000 Kd. The assemb...

example 8

Production of a Useful Plant Extract Containing Immunoglobulins Having a Protection Protein

[0335] Plant pieces (either leaf, stem, flower, root, or combinations) from plants producing immunoglobulins containing a protection protein were mixed with homogenization buffer (2 milliliter buffer per gram of plant material; homogenization buffer: 150 mM NaCl, 20 mM Tris-Cl, pH 7.5), homogenized into a pulp using a Waring blender and centrifuged at 10,000.times. g to remove debris. The supernatant was then extracted with an equal volume of HPLC-grade ethyl acetate by shaking at room temperature, followed by centrifugation at 10,000.times. g. The aqueous phase was transferred to another container, remaining ethyl acetate was removed from the aqueous phase by placing the solution under vacuum. The resulting crude extract consistently contained 100 .mu.g immunoglobulin having a protection protein per ml. This method is useful for any plant containing an immunoglobulin having a protection prote...

example 9

Stability of an Immunoglobulin Containing a Protection Protein

[0338] Two sets of crude plant extracts were prepared as described above. The first extract was derived from a plant expressing an IgG1 antibody and the second extract was derived from a plant expressing an immunoglobulin containing a protection protein. Crude plant extracts of this type from plants are known to contain a variety of proteolytic enzymes. Prolonged incubation of extracts at room temperature or at 37.degree. C. therefore constitutes a proteolytic digestion.

[0339] Using ELISA the quantity of gamma-kappa complexes in the two extracts was determined as a function of time at both room temperature and 37.degree. C. In these assays, an anti-kappa chain antibody was used to coat the plate followed by incubation with the plant extract at 37.degree. C. for 1 hour. An anti-gamma chain antibody conjugated to HRPO was used for detection of immunoglobulin derived from the plant. The quantity of immunoglobulin having a pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
speedsaaaaaaaaaa
pHaaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The immunoglobulins of the present invention are useful therapeutic immunoglobulins against mucosal pathogens such as S. mutans. The immunoglobulins contain a protection protein that protects the immunoglobulins in the mucosal environment. The invention also includes the greatly improved method of producing immunoglobulins in plants by producing the protection protein in the same cell as the other components of the immunoglobulins. The components of the immunoglobulin are assembled at a much improved efficiency. The method of the invention allows the assembly and high efficiency production of such complex molecules. The invention also contemplates the production of immunoglobulins containing protection proteins in a variety of cells, including plant cells, that can be selected for useful additional properties. The use of immunoglobulins containing protection proteins as therapeutic antibodies against mucosal and other pathogens is also contemplated.

Description

[0001] This is a continuation of application Ser. No. 09 / 312,157 filed May 14, 1999, now U.S. Pat. No. 6,303,341, which is a continuation of application Ser. No. 08 / 434,000 filed May 4, 1995 now U.S. Pat. No. 6,046,037 which is a continuation-in-part of application Ser. No. 08 / 367,395 filed Dec. 30, 1994, now abandoned, each of which is hereby incorporated by reference in its entirety including drawings.[0002] The present invention relates to expression of immunoglobulins in plants that contain a protection protein as well as to transgenic plants that express such immunoglobulins. The therapeutic use of these immunoglobulins is also contemplated.BACKGROUND TO THE INVENTION[0003] Monoclonal antibodies have great potential for numerous therapeutic purposes. The advantages of monoclonal antibody therapeutics over conventional pharmaceuticals include their exquisite selectivity, multiple effector functions, and ease of molecular manipulation such as radio-isotope labelling and other typ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/09C07K14/705C07K16/00C07K16/12C07K19/00C12N5/10C12N15/12C12N15/82C12P21/08C12R1/91
CPCC07K14/705C12N15/8258C12N15/8242C07K16/00
Inventor HIATT, ANDREW C.HEIN, MICH B.
Owner THE SCRIPPS RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap