Unlock instant, AI-driven research and patent intelligence for your innovation.

Methods and compositions for detecting signals in binding assays

a technology of binding assay and signal detection, applied in the direction of microorganism testing/measurement, material analysis, biochemistry apparatus and processes, etc., can solve the problems of background noise, time and labor requirements, lack of specificity,

Inactive Publication Date: 2005-03-03
AFFYMETRIX INC
View PDF21 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0004] Methods and compounds are provided for detecting target molecules using specific binding assays. Metho

Problems solved by technology

Methods for the detection of nucleic acid sequences have suffered from drawbacks including background noise, time and labor requirements, lack of specificity and lack of sensitivity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods and compositions for detecting signals in binding assays
  • Methods and compositions for detecting signals in binding assays
  • Methods and compositions for detecting signals in binding assays

Examples

Experimental program
Comparison scheme
Effect test

examples

[0074] A schematic of the current Affymetrix GeneChip® Expression Array signal amplification assay is shown below. Signal amplification over the signal obtained with the first streptavidin-phycoerythrin (SAPE) stain is achieved via a biotinylated anti-streptavidin antibody (b-Ab) that can complex with the SAPE from the first stain. The antibody contains multiple sites where multiple SAPE molecules can bind in the 3rd staining step.

[0075] Affymetrix GeneChip® Expression Array Signal Amplification:

[0076] probe:target-biotin=SAPE=biotinylated anti-streptavidin antibody (b-Ab)=SAPE [0077] step 1—complex biotinylated target:probe duplex with streptavidin-phycoerythrin (SAPE) [0078] step 2—complex biotinylated anti-streptavidin antibody to complex in step 1 [0079] step 3—complex multiple SAPE to complex in step 2

[0080] Microsphere particles were designed to be conjugated with the biotinylated anti-streptavidin antibody used in the current Affymetrix GeneChip® Expression Array signal am...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the presently claimed invention provides a method of labeling a hybridized target wherein the target comprises a binding ligand. In another embodiment, the presently claimed invention provides methods of signal amplification.

Description

PRIORITY CLAIM [0001] This application is a divisional of U.S. application Ser. No. 09 / 687,932, filed on Oct. 13, 2000, which is incorporated by reference for all purposes.BACKGROUND OF THE INVENTION [0002] This invention relates generally to methods and compounds for the detection of polymers and for amplifying detectable signals in specific binding assays, particularly nucleic acid hybridization assays. Nucleic acid hybridizations are commonly used in biochemical research and diagnostic assays. Generally a single stranded analyte nucleic acid is hybridized to labeled nucleic acid probe, and resulting nucleic acid duplexes are detected. Radioactive and nonradioactive labels have been used. Methods also have been developed to amplify the signal that is detected. [0003] Methods for the detection of nucleic acid sequences have suffered from drawbacks including background noise, time and labor requirements, lack of specificity and lack of sensitivity. It is an object of the invention t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
CPCC12Q1/6804Y10S530/81Y10S436/809Y10T436/143333Y10S436/80C12Q2563/149
Inventor LYON, WILLIAM A.TRAN, HUU MINH
Owner AFFYMETRIX INC