Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
Patent Information
- Authority / Receiving Office
- US · United States
- Current Assignee / Owner
- NUGEN TECH
- Publication Date
- 2005-09-22
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application No. 60 / 533,381, filed on Dec. 29, 2003, the disclosure of which is incorporated herein by reference in its entirety.TECHNICAL FIELD
[0002] The invention relates to methods for analysis of nucleic acid methylation status, and methods for fragmentation and / or labeling and / or immobilization of nucleic acids. More particularly, the invention relates to methods for fragmentation and / or labeling and / or immobilization of nucleic acids comprising labeling and / or cleavage and / or immobilization at abasic sites. BACKGROUND
[0003] Methylation of DNA is involved in both normal and abnormal cellular processes. For example, DNA methylation has been implicated in X-inactivation, genomic imprinting, and differential gene expression (such as by upregulation or silencing of genetic loci). DNA methylation plays a role in gene inactivation, cell differentiation, tumorigenesis, X-chromosome ...