Use of a nitroxide or a prodrug thereof in the prophylactic and therapeutic treatment of cancer
a cancer and prophylactic treatment technology, applied in the field of nitroxides and prodrugs thereof, can solve the problems of afflicted suffering physically, emotionally and financially, early death of patients, and major world-wide health problems
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example 1
[0034] This example demonstrates that administration of a nitroxide to p53− / − mice delays the onset of tumors.
[0035] Male and female p53− / − mice (strain 129 / Sv-Trp5ntml Tyi) were purchased from Jackson Labs (Bar Harbor, Me.). Such animals uniformly die within a few months after birth due to rapid tumor formation and growth. Animals arrived in the laboratory at 7-8 weeks of age, were acclimated for five days and were randomly divided into control (n=8; average weight=24.6 g) and treatment (n=9; average weight=25.0 g) groups. Both groups were allowed food and water ad libitum. The water of the control group was supplemented with sugar (4 g / 100 ml), whereas the water of the treatment group was supplemented with sugar (4 g / 100 ml) and 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (Tempol) to a final concentration of 58 mM. Mice were sacrificed at the first sign of a visible tumor nodule, gross enlargement of the spleen or marked difficulty in breathing. The results are shown in FIG. 1...
example 2
[0036] This example demonstrates that administration of a nitroxide to normal C3H female mice for their entire life-span decreases the incidence of cancer in such mice.
[0037] Female C3H mice were supplied through the Frederick Cancer Research Center-Animal Production, Frederick, Md. Animals were received at 6 weeks of age and were randomly divided into groups (n=20 / group) as follows: Control-1, which received regular food and water; Control-2, which received regular food and water supplemented with sugar (4 g / 100 ml); Tempol / I Year, which received regular food and water supplemented with sugar (4 g / 100 ml) and Tempol to a final concentration of 58 mM for one year, after which they were converted to regular food and water; and Tempol / Entire Life Span, which received regular food and water supplemented with sugar (4 g / 100 ml) and Tempol to a final concentration of 58 mM for their entire life span. All groups were allowed food and water ad libitum. All groups were followed for their e...
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