Viral detection liposomes and method

a technology of liposomes and viral pathogens, applied in the field of viral detection liposomes and methods, can solve the problems of laborious and complicated detection and assay of viral pathogens

Inactive Publication Date: 2011-02-10
NANOTROPE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach enhances detection speed, accuracy, and sensitivity, allowing for simultaneous detection of multiple pathogens and providing a robust, sensitive method for analyzing viral RNA, with the ability to capture and analyze genetic material for further verification and potential vaccine development.

Problems solved by technology

The detection and assaying of viral pathogens is very complicated and labor intensive.

Method used

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  • Viral detection liposomes and method
  • Viral detection liposomes and method
  • Viral detection liposomes and method

Examples

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Embodiment Construction

[0015]The disclosed novel detection methods for a viral identification assay are based on mimicking the response mechanisms similar to those found in living cells. By tailoring or engineering liposomes with the appropriate biomolecular and biochemical compositions these particles are comprised of the necessary biological elements to sense and identify potential biological threats. The application of nanodroplet technology provides customized liposomes that are packaged with the suitable binding substrates, biochemical reagents, and optical reporters. These liposomes are functionalized with pre-determined surface receptors that specifically target conserved characteristics of unknown or engineered pathogens of certain biological classes. Due to the flexible nature of the nanodroplet technology for providing various liposome compositions on demand, this approach has significant impact in the rapid development and deployment of reagents and receptor molecules for integration into exist...

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Abstract

A method of generating pathogen detecting liposomes includes a step of providing molecular beacons with fluorescing components. The molecular beacons include either strands of RNA or DNA and the fluorescing components include an emitter and a quencher. The method further uses nanodroplet technology to encapsulate the molecular beacons within a lipid membrane. Subsequently, receptors are assembled in association with the membrane.

Description

FIELD OF THE INVENTION[0001]This invention relates to specifically engineered liposomes and methods of use for the detection of viral pathogens and the like including unknown, mutated, or engineered varieties.BACKGROUND OF THE INVENTION[0002]The detection and assaying of viral pathogens is very complicated and labor intensive. At a time when new and different viral pathogens appear regularly and with increasing frequency, the detection and identification, as well as improved methods and apparatus for collection, as quickly and efficiently as possible are highly desirable. This of course is true for mutated and engineered varieties as well as any unknown varieties. At the present time, the only known method for detection and identification is by processing collected viral pathogens in a laboratory using well known testing or trial-and-error procedures such as cell cultures or PCR.[0003]It would be highly advantageous, therefore, to remedy the foregoing and other deficiencies inherent...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): C12Q1/70C07H21/04
CPCY10T428/2984A61K31/711
InventorTU, EUGENEACKLEY, DONALD E.FORSTER, ANITAKRIHAK, MICHAEL
OwnerNANOTROPE