Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Circulating microrna as a marker for hepatocellular carcinoma

a circulating microrna and hepatocellular carcinoma technology, applied in the direction of instruments, biochemistry apparatus and processes, material analysis, etc., can solve the problems of variable effectiveness of tools, serological studies lacking sensitivity and specificity in patients with small tumors

Inactive Publication Date: 2011-09-15
QUEST DIAGNOSTICS INVESTMENTS INC
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is based on the discovery of circulating miRNAs in patient samples, particularly in body fluid samples such as serum or plasma. The levels of certain miRNAs, such as miR-16, miR-195, and miR-199a, are measured and compared to reference levels from healthy individuals or chronic liver disease patients. The levels of these miRNAs can be used to diagnose and prognose hepatocellular carcinoma (HCC) in a subject. The invention also provides methods for monitoring the effectiveness of anti-cancer therapy in patients diagnosed with HCC. The levels of miRNAs and other biomarkers, such as AFP, AFP-L3, and DCP, are measured and used to determine the presence of HCC in a subject.

Problems solved by technology

These diagnostic tools have variable effectiveness for early diagnosis of HCC; cross-sectional imaging typically detects only tumors greater than 1 cm in diameter, and serological studies also lack sensitivity and specificity in patients with small tumors.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Circulating microrna as a marker for hepatocellular carcinoma
  • Circulating microrna as a marker for hepatocellular carcinoma

Examples

Experimental program
Comparison scheme
Effect test

example

[0092]The present methods and kits, thus generally described, will be understood more readily by reference to the following examples, which are provided by way of illustration and are not intended to be limiting of the present methods and kits. The following is a description of the materials and experimental procedures used in the example.

Materials and Methods

[0093]Study Subjects. A total of 283 subjects were studied. The first group consisted of 105 patients with liver cirrhosis complicated by HCC. The diagnosis of HCC was confirmed by biopsy and histological evaluation or detection of new hepatic lesion with arterial phase enhancement on computed tomography or magnetic resonance imaging. The second group included 107 patients with chronic liver diseases (CLDs) and no HCC. All patients had biopsies reviewed by a pathologist, fibrosis staging between 0 and 4 (metavir scale), and variable degrees of inflammation. The clinical follow up in this group included ultrasound and AFP measur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Fast real time PCRaaaaaaaaaa
Fast real time PCRaaaaaaaaaa
Login to View More

Abstract

Provided herein are methods for the diagnosis, or management of liver diseases, e.g., hepatocellular carcinoma, using profiles of the miRNAs determined from cellular or acellular body fluids.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims benefit of U.S. Provisional Application 61 / 313,677, filed Mar. 12, 2010, which is herein incorporated by reference in its entirety.FIELD OF THE INVENTION[0002]The invention generally relates to the diagnosis, prognosis, and / or management of liver diseases including hepatocellular carcinoma.BACKGROUND OF THE INVENTION[0003]The following discussion of the background of the invention is merely provided to aid the reader in understanding the invention and is not admitted to describe or constitute prior art to the present invention.[0004]Hepatocellular carcinoma (HCC), the fifth most common solid tumor in the world, has shown a rising trend in terms of both incidence and mortality in the United States. These increases are largely associated with cirrhosis due to chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infection, alcohol abuse, and other causes. Current diagnosis of HCC in patients with clinical suspi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68G01N33/48
CPCC12Q1/6886G01N2333/471G01N33/57438C12Q2600/178
Inventor QU, KEVINZHANG, KEALBITAR, MAHER
Owner QUEST DIAGNOSTICS INVESTMENTS INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com