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Assay for the Rapid Detection of Nucleic Acids via a Modified LAMP Reaction Coupled with Colorimetric Reporter Utilizing a Gold Nanoparticle Peptide Nucleic Acid AuNP-PNA Probe System

a reporter and nucleic acid technology, applied in the field of rapid detection of nucleic acids via modified lamp reaction coupled with colorimetric reporter utilizing gold nanoparticle peptide nucleic acid probe system, can solve problems such as false positives, and achieve the effect of superior specificity of aunp/pna probe system and rapid diagnostic assay

Inactive Publication Date: 2020-07-16
MANN PAUL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention is an improved way to detect nucleic acids (DNA / RNA) using a special amplification technology and a color change detection method. It combines specific amplification with specific detection, resulting in a more accurate and reliable assay. This is the first time this approach has been attempted, and it is believed to be more accurate than other color change detection methods.

Problems solved by technology

While convenient, these reporter systems do not detect the presence of a specific nucleic acid sequence and thus have the potential for false positives.

Method used

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  • Assay for the Rapid Detection of Nucleic Acids via a Modified LAMP Reaction Coupled with Colorimetric Reporter Utilizing a Gold Nanoparticle Peptide Nucleic Acid AuNP-PNA Probe System
  • Assay for the Rapid Detection of Nucleic Acids via a Modified LAMP Reaction Coupled with Colorimetric Reporter Utilizing a Gold Nanoparticle Peptide Nucleic Acid AuNP-PNA Probe System
  • Assay for the Rapid Detection of Nucleic Acids via a Modified LAMP Reaction Coupled with Colorimetric Reporter Utilizing a Gold Nanoparticle Peptide Nucleic Acid AuNP-PNA Probe System

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Embodiment Construction

[0020]As described herein with reference to FIGS. 1-4, the invention successfully performs the assay described below on both a viral pathogen (HIV) and a bacterial pathogen (Staphylococcus aureus).

[0021]The invention exhibits several aspects of novelty as follows:

[0022]1) Improved specificity over a traditional LAMP assay.

[0023]2) Modification of LAMP primers for the generation of a target dependent sequence (FIG. 1).

[0024]3) Colorimetric detection of the target dependent sequence via a AuNP / PNA (gold nanoparticle / peptide nucleic acid) probe system (FIGS. 2 and 4).

[0025]4) It is believed that this is the first colorimetric assay to be specific for detection of a LAMP product. Previous colorimetric methodologies are indirect measures of LAMP amplification.

[0026]The invention relates to an assay and method developed as a rapid diagnostic assay for detection of nucleic acids (DNA / RNA). The process utilizes isothermal nucleic acid amplification technology coupled with a gold nanoparticl...

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Abstract

An assay is provided for the rapid detection of nucleic acids via a modified LAMP reaction coupled with colorimetric reporter utilizing a gold nanoparticle-peptide nucleic acid (AuNP-PNA) probe system.

Description

FIELD OF THE INVENTION[0001]The invention relates to an assay for the rapid detection of nucleic acids via a modified LAMP reaction coupled with colorimetric reporter utilizing a gold nanoparticle-peptide nucleic acid (AuNP-PNA) probe system.BACKGROUND OF THE INVENTION[0002]An isothermal molecular technique known as Loop Mediated Isothermal Amplification (LAMP) allows for amplification and detection of specific nucleic acid sequences. LAMP is a culture-independent nucleic acid amplification technique that is more specific and sensitive than traditional polymerase chain reaction (PCR). LAMP assays have been adapted for rapid detection (less than 30 minutes) of nucleic acids and can be performed without nucleic acid extraction steps, making this technology highly amenable for point-of-care assays. Colorimetric LAMP assays allow the user to detect a potentially positive result as a result of a color change in the reaction vessel. Several colorimetric reporter systems have been used to ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6853C12Q1/6806C12Q1/6869C12Q1/6816
CPCC12Q2600/158C12Q1/6816C12Q1/6853C12Q1/6806C12Q2600/156C12Q2600/16C12Q1/6869C12Q1/6844C12Q1/703C12Q2521/107C12Q2525/107C12Q2531/119C12Q2563/137
Inventor MANN, PAULJENNINGS, MATTHEW
Owner MANN PAUL